Summary
Overview
Work History
Education
Skills
Languages
Certification
Websites
Timeline
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VALERIO EMBRIONE

San Diego,CA

Summary

  • PhD Scientist with 3 years of industry experience and a total of 17 years of experience in life sciences;
  • Experience with molecular, cellular, in-vivo and in-silico techniques, including CRISPR-Cas9, NGS and protein assays;
  • Certified knowledge of all the steps of drug discovery, translational medicine and clinical trials;
  • Successfully led projects on NASH, oncology and neurodegenerative diseases;
  • Leadership abilities with experience in supervising and coaching workforce.

Overview

18
18
years of professional experience
1
1
Certification

Work History

Scientist

Viscient Biosciences
01.2021 - 11.2023
  • Independently developed a platform for gene regulation (overexpression and RNAi) in 3D primary liver organoids for drug discovery;
  • Project leader for the discovery of 4 novel targets in liver inflammatory diseases;
  • Leader of the bioinformatics operations, including 3 successfully delivered projects in collaboration with CROs;
  • Coached and supervised 3 research associates;
  • Co-author of 1 peer-reviewed paper (submitted), 2 posters for international scientific conferences and 1 (pending) patent application.

Clinical Laboratory Specialist

The Ohio State University
03.2020 - 12.2020
  • Trained on BSL3 setting and CLIA guidelines;
  • Shift leader for COVID-19 patients samples collection, preparation, and molecular and quick tests improving results turnaround time by over 30%;
  • Supervised a team of 12 total clinical lab associates and volunteers.

Graduate Research Associate

The Ohio State University
08.2014 - 12.2020
  • Generated customized cell lines of human Triple Negative Breast Cancer using CRISPR-Cas9 technology;
  • Performed Next Generation Sequencing (NGS) design and analysis for microRNA processing;
  • Generated and characterized 5 recombinant proteins;
  • Generated and analyzed a murine model for lung cancer study;
  • First author of 1 peer-reviewed paper, co-authored of 1 peer-reviewed paper, 1 oral presentation at international conference and author/co-author of 5 posters for international scientific conferences;
  • Supervised 1 research associate, 1 research assistant and 4 undergraduate research associates.

Research Associate

The Ohio State University
01.2013 - 07.2014
  • Generated and analyzed mouse models of multiple mutations in 3 different organs at different developmental stages;
  • Trained on mouse surgery and organ electroporation according to IACUC regulations;
  • Authored/co-authored 3 posters for international scientific conferences;
  • Coached and supervised 1 research assistant and 3 undergraduate research associates.

Research Fellow

Italian Institute Of Technology (IIT)
01.2012 - 12.2012
  • Generated a platform for the analysis of cell growth on different materials;
  • Led experimental plans with external collaborators;
  • Co-authored 4 scientific publications and 3 posters for international scientific conferences.

Clinical Laboratory Technician

Orio Clinical Center
01.2010 - 12.2011
  • Collected and analyzed human samples for biochemistry, hematology, and microbiology diagnostic tests.

Graduate Research Assistant

Telethon Institute Of Genetics And Medicine
01.2006 - 08.2009
  • Trained on multiple protein, DNA and RNA molecular techniques;
  • Trained on bioinformatics tools for the discovery of novel transcription factor binding sites;
  • Co-authored 1 scientific publication on major journal and 4 posters for international scientific conferences.

Education

Ph.D. - Molecular, Cellular And Developmental Biology

The Ohio State University
Columbus, OH
12.2020

Master of Science - Medical Biotechnology

University of Naples Federico II
Naples, Italy
06.2011

Bachelor of Science - Biotechnology

University of Naples Federico II
Naples, Italy
03.2007

Skills

  • Murine models manipulation according to IACUC protocols (husbandry, surgery, necropsy, tail vein injection)
  • DNA assays (Purification, Electrophoresis, Sequencing, Cloning)
  • RNA assays (Purification, Electrophoresis, In-vitro transcription)
  • NGS library generation and bioinformatics analysis
  • Immunoprecipitation (IP/Co-IP, RIP, ChIP)
  • CRISPR/Cas9 and Degron
  • 2D and 3D (organoids) primary and immortalized cell culture
  • Cell based assays (Viability, apoptosis, mobility)
  • Cell transfection (plasmids, RNAi) and lentiviral transduction
  • PCR/qPCR/RT-PCR
  • Protein assays (Bradford assay, Western Blot, ELISA, protein purification)
  • Flow Cytometry/FACS
  • Immunohistochemistry (IHC)
  • Immunofluorescence (IF)
  • FlowJo, GraphPad Prism and Microsoft Office
  • Benchling, PyMol and docking and modeling tools
  • Database analysis from cBioPortal/TCGA, GEO, UCSC Genome Browser

Languages

English
Full Professional
Italian
Native or Bilingual
Spanish
Limited Working

Certification

  • Drug Development Product Management Specialization - University of California San Diego
  • Clinical Trials Operation Specialization - Johns Hopkins University
  • Translational Science Specialization - University of Michigan

Timeline

Scientist

Viscient Biosciences
01.2021 - 11.2023

Clinical Laboratory Specialist

The Ohio State University
03.2020 - 12.2020

Graduate Research Associate

The Ohio State University
08.2014 - 12.2020

Research Associate

The Ohio State University
01.2013 - 07.2014

Research Fellow

Italian Institute Of Technology (IIT)
01.2012 - 12.2012

Clinical Laboratory Technician

Orio Clinical Center
01.2010 - 12.2011

Graduate Research Assistant

Telethon Institute Of Genetics And Medicine
01.2006 - 08.2009

Ph.D. - Molecular, Cellular And Developmental Biology

The Ohio State University

Master of Science - Medical Biotechnology

University of Naples Federico II

Bachelor of Science - Biotechnology

University of Naples Federico II

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