VALERIO EMBRIONE
San Diego,CA
Summary
- PhD Scientist with 3 years of industry experience and a total of 17 years of experience in life sciences;
- Experience with molecular, cellular, in-vivo and in-silico techniques, including CRISPR-Cas9, NGS and protein assays;
- Certified knowledge of all the steps of drug discovery, translational medicine and clinical trials;
- Successfully led projects on NASH, oncology and neurodegenerative diseases;
- Leadership abilities with experience in supervising and coaching workforce.
Overview
18
18
years of professional experience
1
1
Certification
Work History
Scientist
Viscient Biosciences
2021.01 - 2023.11
- Independently developed a platform for gene regulation (overexpression and RNAi) in 3D primary liver organoids for drug discovery;
- Project leader for the discovery of 4 novel targets in liver inflammatory diseases;
- Leader of the bioinformatics operations, including 3 successfully delivered projects in collaboration with CROs;
- Coached and supervised 3 research associates;
- Co-author of 1 peer-reviewed paper (submitted), 2 posters for international scientific conferences and 1 (pending) patent application.
Clinical Laboratory Specialist
The Ohio State University
2020.03 - 2020.12
- Trained on BSL3 setting and CLIA guidelines;
- Shift leader for COVID-19 patients samples collection, preparation, and molecular and quick tests improving results turnaround time by over 30%;
- Supervised a team of 12 total clinical lab associates and volunteers.
Graduate Research Associate
The Ohio State University
2014.08 - 2020.12
- Generated customized cell lines of human Triple Negative Breast Cancer using CRISPR-Cas9 technology;
- Performed Next Generation Sequencing (NGS) design and analysis for microRNA processing;
- Generated and characterized 5 recombinant proteins;
- Generated and analyzed a murine model for lung cancer study;
- First author of 1 peer-reviewed paper, co-authored of 1 peer-reviewed paper, 1 oral presentation at international conference and author/co-author of 5 posters for international scientific conferences;
- Supervised 1 research associate, 1 research assistant and 4 undergraduate research associates.
Research Associate
The Ohio State University
2013.01 - 2014.07
- Generated and analyzed mouse models of multiple mutations in 3 different organs at different developmental stages;
- Trained on mouse surgery and organ electroporation according to IACUC regulations;
- Authored/co-authored 3 posters for international scientific conferences;
- Coached and supervised 1 research assistant and 3 undergraduate research associates.
Research Fellow
Italian Institute Of Technology (IIT)
2012.01 - 2012.12
- Generated a platform for the analysis of cell growth on different materials;
- Led experimental plans with external collaborators;
- Co-authored 4 scientific publications and 3 posters for international scientific conferences.
Clinical Laboratory Technician
Orio Clinical Center
2010.01 - 2011.12
- Collected and analyzed human samples for biochemistry, hematology, and microbiology diagnostic tests.
Graduate Research Assistant
Telethon Institute Of Genetics And Medicine
2006.01 - 2009.08
- Trained on multiple protein, DNA and RNA molecular techniques;
- Trained on bioinformatics tools for the discovery of novel transcription factor binding sites;
- Co-authored 1 scientific publication on major journal and 4 posters for international scientific conferences.
Education
Ph.D. - Molecular, Cellular And Developmental Biology
The Ohio State University
Columbus, OH12.2020
Master of Science - Medical Biotechnology
University of Naples Federico II
Naples, Italy06.2011
Bachelor of Science - Biotechnology
University of Naples Federico II
Naples, Italy03.2007
Skills
- Murine models manipulation according to IACUC protocols (husbandry, surgery, necropsy, tail vein injection)
- DNA assays (Purification, Electrophoresis, Sequencing, Cloning)
- RNA assays (Purification, Electrophoresis, In-vitro transcription)
- NGS library generation and bioinformatics analysis
- Immunoprecipitation (IP/Co-IP, RIP, ChIP)
- CRISPR/Cas9 and Degron
- 2D and 3D (organoids) primary and immortalized cell culture
- Cell based assays (Viability, apoptosis, mobility)
- Cell transfection (plasmids, RNAi) and lentiviral transduction
- PCR/qPCR/RT-PCR
- Protein assays (Bradford assay, Western Blot, ELISA, protein purification)
- Flow Cytometry/FACS
- Immunohistochemistry (IHC)
- Immunofluorescence (IF)
- FlowJo, GraphPad Prism and Microsoft Office
- Benchling, PyMol and docking and modeling tools
- Database analysis from cBioPortal/TCGA, GEO, UCSC Genome Browser
Languages
English
Full Professional
Italian
Native or Bilingual
Spanish
Limited Working
Certification
- Drug Development Product Management Specialization - University of California San Diego
- Clinical Trials Operation Specialization - Johns Hopkins University
- Translational Science Specialization - University of Michigan
Timeline
Scientist
Viscient Biosciences
2021.01 - 2023.11
Clinical Laboratory Specialist
The Ohio State University
2020.03 - 2020.12
Graduate Research Associate
The Ohio State University
2014.08 - 2020.12
Research Associate
The Ohio State University
2013.01 - 2014.07
Research Fellow
Italian Institute Of Technology (IIT)
2012.01 - 2012.12
Clinical Laboratory Technician
Orio Clinical Center
2010.01 - 2011.12
Graduate Research Assistant
Telethon Institute Of Genetics And Medicine
2006.01 - 2009.08
Ph.D. - Molecular, Cellular And Developmental Biology
The Ohio State University
Master of Science - Medical Biotechnology
University of Naples Federico II
Bachelor of Science - Biotechnology
University of Naples Federico II