Vincent Vui King Chong
Buffalo,New York
Summary
A cancer researcher with expertise in molecular and cellular biology, non-invasive imaging, and preclinical cancer models involved in drug discovery and development. Research interests focus on understanding drug resistance mechanisms and developing novel mechanism-based cancer treatments. Have experience as a project leader, with laboratory managerial competencies and a track record of successful collaboration and publications in top-tier journals (total 28 of them). With analytical skills, attention to detail, and critical thinking make them a valuable asset to any research team focused on improving cancer treatment options.
Overview
14
14
years of professional experience
Work History
Graduate School Associate Member
University At Buffalo
Buffalo, NY
04.2023 - Current
- Mentoring and supervised graduate student for experiments and preparing manuscript and thesis.
- Led and prepared lecturers for cancer biology and research and methodology courses including in person and online.
- Simultaneously managed several research projects in lab as well as collaborations with other researchers globally.
- Generated grant proposals and gain funding for research from federal and states agencies.
Project Scientist (Affiliate Member)
Roswell Park Comprehensive Cancer Center
Buffalo, NY
08.2021 - Current
- Development of novel clinically relevant models of oral/head and neck cancers such as primary monolayer, spheroid and organoid (in vitro) and PDX, syngeneic and humanized mouse models
- Translational investigation of disease mechanisms in head and neck preclinical models by investigating essential cancer pathways that related to DNA repair, cell cycle, EGFR signaling that related to PI3K/ERK/AKT/STAT3 pathways.
- High-throughput drug screenings in collaborations with drug screening core facilities by screening FDA approved drug library with 1506 compounds in organoid models.
- Non-invasive imaging such as magnetic resonance imaging monitor longitudinally of tumor growth in vivo and bioluminescent imaging such as luciferin to detect longitudinally of tumor growth and using firefly luciferase (Fluc) and luminol in tumor of in vivo.
- Developed organoid -based assays to identify the mechanistic role of various targets in cancer using small molecule that inhibit the target expression and determine the therapeutic response to standard of care treatment.
- Identify novel therapeutic agents using drug repurposing approach with and without standard of care regimens in preclinical models.
- Developed organoid-immune cell (CD3 and CD8 T cell) co-culture assay to evaluate the immunomodulatory efficacy of lead compound using ELISA and validate using syngeneic or humanized head and neck cancer model through flow cytometry.
- Utilized flow cytometry for immune-phenotyping (10 fluorochrome colour panel) to measure T cell functionality such as IFNG and Granzyme B.
- Performed several immunoassays to analyze the cytokine levels in cell cultures supernatant in response to various treatments.
- Analyzed post-transcriptional and translational effect after treatment by measuring gene expression via qPCR and protein levels by western blot.
- Develop stable knock out cell lines using either plasmid transfection or lentivirus transduction.
- Evaluated and implemented CyTOF and Nanostring spatial transcriptomic sequencing.
- Resulted in four publications through collaboration with other scientists, which relate to the translational effects of using small molecule therapeutic agents in cancer preclinical models.
Project Scientist (Postdoctoral Research Fellow)
Roswell Park Comprehensive Cancer Center
Buffalo, NY
01.2017 - 07.2021
- Understanding the mechanisms involved in oral carcinogenesis using chemoprevention approach such as determine the tumor burden through histology assessment and MRI segmentation.
- Maintained accurate records of research findings and provided statistical analysis of data results.
- Contributed to and actively participated in research conception, design and execution to address defined problems.
- Resulted in 6 publications (4 first author and 2 as co-authors) that involved with chemoprevention and therapeutic approach in preclinical model of cancer.
PhD Scholar
University Of Malaya
Kuala Lumpur
03.2009 - 12.2016
- Characterized the oncogenic roles of MMP13 and ISG15 as potential driver genes in in vitro through cell proliferation, migration, invasion, and apoptosis assays.
- Characterized the clinical roles of MMP13 and ISG15 as predictor for pathological stages, lymph node metastasis and prognosticator in oral cancer using immunohistochemistry technique.
- Collaborate with clinician, oncologist, clinical scientists, tissue procurement officer to execute and deliver translational and biomarker strategy using clinical samples
- Authored 18 manuscript with 3 first author and remaining is first co-author or significant authors.
Education
Ph.D. - Oncology
University of Malaya
Malaysia02.2017
Master of Dental Science - Molecular Cytogenetic
University of Malaya
Malaysia03.2012
Bachelor of Science - Biomedical Science
University of Tunku Abdul Rahman
Malaysia10.2008
Skills
Cell Biology Assays and Techniques: Mammalian Cell Culture (Monolayer, Spheroid and Organoid) and Cell Based Assays (Cell Viability, Apoptosis, Clonogenicity, Migration and Invasion Assays), Drug Screening, Co-Culture, T Cell Isolation, Flow Cytometry, Microcopy and Fluorescent Microscopy
Molecular Biology Techniques: DNA Extraction, RNA Extraction, Protein Extraction, Wester Blotting, PCR, qPCR, Single Cell Preparation, siRNA Knockdown, SgRNA Knockout, RNA Sequencing, Transfection, Transduction
Histology: Cryosection, Immunohistochemistry, Immunofluorescence, FISH
Animal Models: Extensive experience in working with Syngeneic, Cell Derived and Patient Derived Mouse Model of Cancer including Cell/Tissue Implantation, Survival Surgery, Drug Treatment (IP/PO/IV), Blood Collection, And Tissue Harvesting, Animal MRI Imaging, Bioluminescent Imaging, Radiation
Data Analyses Tools and Software: Proficient in Working with Microsoft Word, Excel, Power Point, Graphpad Prism, SPSS, Image J, Compusyn Softwre, Winlist for Flow Cytometry Analysis, Biostatistical and Other Data Analyses
Professional: Mentoring, Project Management, Team Leadership, Group Discussions, Problem Solving, Writing Scientific Papers, Data Management, Organization and Prioritization, Critical Thinking, Creative Thinking, Communication, Team Work
Affiliations
Presentations
- Vincent-Chong VK et al. Patient-derived organoids recapitulate response heterogeneity in head and neck cancer. AACR-AHNS Head and Neck Cancer Conference 2019 April 29-30.
- Vincent-Chong VK et al. Deciphering radiation resistance in head and neck cancer using patient derived organoids. AACR Virtual Special Conference on Radiation Science and Medicine; 2021 Mar 2-3.
Publications
- Vincent-Chong VK, Seshadri M. Development and Radiation Response Assessment in A Novel Syngeneic Mouse Model of Tongue Cancer: 2D Culture, 3D Organoids and Orthotopic Allografts. Cancers (Basel). 2020 Mar 2;12(3):579.
- Vincent-Chong VK, DeJong H, Attwood K, Hershberger PA, Seshadri M. Preclinical Prevention Trial of Calcitriol: Impact of Stage of Intervention and Duration of Treatment on Oral Carcinogenesis. Neoplasia. 2019 Apr;21(4):376-388
Timeline
Graduate School Associate Member
University At Buffalo
04.2023 - Current
Project Scientist (Affiliate Member)
Roswell Park Comprehensive Cancer Center
08.2021 - Current
Project Scientist (Postdoctoral Research Fellow)
Roswell Park Comprehensive Cancer Center
01.2017 - 07.2021
PhD Scholar
University Of Malaya
03.2009 - 12.2016
Ph.D. - Oncology
University of Malaya
Master of Dental Science - Molecular Cytogenetic
University of Malaya
Bachelor of Science - Biomedical Science
University of Tunku Abdul Rahman
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