WANNAPORN ITTIPRASERT TANNO
Clarksville,MD
Summary
Skilled Researcher enthusiastic about supporting advancements in molecular biology and immunology especially infectious diseases causing cancer. Passionate about increasing knowledge to drive growth and needed improvements. Ready to apply knowledge and 20 years of experience in translation and clinical research.
Overview
22
22
years of professional experience
Work History
Research Associate Faculty
George Washington University
01.2017 - Current
- Designed, Planed, and in vitro investigated mRNA-LNP vaccine candidate for infectious diseases
- Contributed to siRNA delivery platform for cancer therapy in mouse model
- Conducted new transgenic commensal organisms to secrete chimeric nanobody (VHH-Fc) neutralizing human virus
- Developed genetic engineering for transgene integration efficiency from 2% up to 70% by multiplex CRISPR, modified DNA donor with LNP delivery
- Conducted KO cell lines by lentiviral transduction for tumor microenvironment
- Constructed rodent models for inflammation/granuloma, T helper cells and cancer mechanism in vivo and ex vivo studies
- Spearheaded initiatives to functional genomic helminths, which resulted in transgenesis, gain/loss of function of drug and vaccine candidates in vitro and in vivo rodent models
- Managed 2 projects sponsored by NCI and DoD, oversaw dynamic project teams comprised of senior and junior scientists, and ensured on-time and on-budget completion
- Authored and coauthored multiple papers related to research topics in diagnosis development, immune responses to infections regulated lung and liver granuloma formations and cancer and cancer therapy.
Research Assistant Faculty
George Washington University
12.2015 - 12.2016
- Delivered new initiatives to functional genomic initiative, which resulted in novel transferring gene manipulation in helminths and human cell line
- Communicate/co-ordinate with diverse scientists from Europe, Asia and North America, build rapport and long-term relationships, and achieve external funds for cancer risk factor, identification, drug and vaccine target investigation.
Research Associate
Biomedical Research Institute (BRI)
04.2011 - 11.2015
- Led and manufactured of biological and molecular reagents for research community
- Managed preparation documentation for BEI Resources, ATCC submissions and QC forms including study protocols
- Delivered molecular biology tools for the schistosomiasis research community and the development of optimized protocols
- Coordinate with coordinators to ensure the accuracy and transparency of data.
Postdoctoral Fellow
Biomedical Research Institute (BRI)
01.2006 - 03.2011
- Designed, conducted the fluorescent labeled-siRNA or -dsRNA delivery into invertebrate embryonic cells for gene function study and morphology
- Generated laboratory resistant to human parasite infection to use in biological vector control experiments
- Identified DNA/microsatellite markers for differential schistosomiasis diagnosis
- Contributed and published in the whole genome of invertebrate project.
Scientist
Mahidol University
03.2002 - 11.2005
- Won Postdoctoral Trainee Award in 2004 to be trained at University of Edinburgh, Edinburgh, UK and generated a transgenic mouse for autoimmune disease model
- Constructed KO mice for autoimmune disease to investigate T cell function
- Communicate with non-clinical and clinical research teams, build rapport and long-term relationships for research in gene polymorphism in autoimmune patients.
Education
Doctor of Philosophy - Tropical Medicine&Hygiene, major Immunology
Mahidol University
03.2002
Bachelor of Science - Medical Technology
Mahidol University
03.1997
Skills
- Biochemistry
- Immunological assays
- Cell Biology
- Molecular Biology
- Animal models
- Clinical trials support
- Data analysis
- Reporting Requirements
- Lab Operations
- Research and Analysis
- Inventory Reviews
- Literature Reviews
- Reporting Standards
- Grant Writing
- Experimental Design
- Lab Safety
- Technical Writing
- Project Management
- Progress Reporting
- Research Planning
- EndNote proficiency
- Quantitative research
- Interpersonal skills
- Adaptability
- Data interpretation
- Biostatistics
- Software proficiency
- Qualitative research
- Laboratory techniques
- Decision making
- Protocol development
- Hypothesis testing
- Research methodology
- Technical report writing
- Time management
- Microsoft Office Suite
- Scientific writing
- Multitasking
- Cross-Functional Teamwork
- Documentation and Reporting
- Experiment Design
- Proposal Preparation
- Interpreting Results
Accomplishments
- Royal Golden Jubilee Ph.D. program, Thailand Research Fund 05/1999-03/2002
- Student Award, Southeast Asian Minister of Education Organization 05/1998-04/1999
Expertise Summary
Accomplished Molecular Biologist and Immunologist expertise in infections-induced cancer mechanism using gene editing tools with extensive experience, excelling in both academic and federal contract environments. A proven track record of research accomplishments, underscored by the publication of >40 peer-reviewed articles, attests to a deep understanding of molecular, immunological, host responses and tumor processes. Proficient in conducting, managing, and overseeing research initiatives, combining technical expertise with administrative acumen.
Team Skills
- Active Engagement: Regular participation in lab meetings, university seminars, and multi-collaborative group meetings. Contributed to idea exchange and provided constructive criticisms, fostering a dynamic intellectual environment.
- Efficient Collaboration: Organized successful collaborations by scheduling meetings, setting agendas, and facilitating scientific discussions. Ensured deadlines were met, promoting a collaborative and goal-oriented approach.
- Supervision and Mentorship: Provided leadership by supervising technicians, graduate students, undergraduates, and visitor scientists. Cultivated a supportive environment for skill development and academic growth.
Fundings
- Defense Advanced Research Projects Agency, DoD (co-I) 04/2022-present
- National Cancer Institute, NIH fund (co-I) 07/2017-07/2023
- Wellcome Trust Strategic Award, UK (key person) 12/2015-03/2022
Invited Talks
- Functional Genomics for Tropical Diseases at Instituto René Rachou / FIOCRUZMinas, Belo Horizonte, MG, Brazil in titile 'Genome editing in flatworms for gene functional study', 09/2023
- Annual Graduate Summer Seminar at Biomedical Research Center Seltersberg, Justus Liebig University Giessen, Giessen, Germany in titled 'Gene manipulation by CRISPR-Cas technology for macrobiome therapeutic' 08/2022
- Molecular Biology Application in Infectious Diseases at Biomedical Research Institute, MD in titled 'Gene modified-helminth models for Biomedical Research using CRISPR/Cas technology', 04/2021
Selected Peer Reviewed
- Multiplexed CRISR/Cas9 method for large transgene integration into the Schistosoma mansoni genome, STAR Protocol (in press), Ittiprasert W et al., 2024
- Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni, Cell Reports Methods, Ittiprasert W et al., 2023
- A novel aptamer-based small RNA delivery platform and its application to cancer therapy, Genes and Diseases, Tanno T, Zhang P, Bailey C, Wang Y, Ittiprasert W et al., 2022
- Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini, PLOS Pathogens, Chaiyadet S#, Tangkawattana S#, Smout MJ#, Ittiprasert W# et al., 2022
- RNA-guided AsCas12a- and SpCas9-catalyzed knockout and homology directed repair of the omega-1 locus of the human Blood Fluke, Schistosoma mansoni, International Journal of Molecular Sciences, Ittiprasert W et al., 2022
- An Innovative Test for the rapid detection of specific IgG antibodies in human whole-blood for the diagnosis of Opisthorchis viverrine infection, Tropical Medicine and Infectious Disease, Sadaow L, Rodpai R, Janwan P, … Ittiprasert W et al., 2022
- Tumor Necrosis Factor and Schistosoma mansoni egg antigen omega-1 shape distinct aspects of the early egg-induced granulomatous response, PLOS Neglected Tropical Diseases, Takaki KK, Roca FJ, Schramm G, Wilbers RHP, Ittiprasert W et al., 2021
- Rapid assessment of Opisthorchis viverrini IgG antibody in serum: A potential diagnostic biomarker to predict risk of cholangiocarcinoma in regions endemic for opisthorchiasis, International Journal of Infectious Diseases, Rodpai R, Luvira V, Sadaow L,…Ittiprasert W et al., 2022
- Infection with Helicobacter pylori induces epithelial to mesenchymal transition in human cholangiocytes, Pathogens, Thanaphongdecha P, Karinshak SE, Ittiprasert W et al., 2020
- Diminished adherence of Biomphalaria glabrata embryonic cell line to sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor, Parasite Vectors, Coelho FS, Rodpai R, Miller A, …, Ittiprasert W
- 2020
- Liver fluke granulin promotes extracellular vesicle-mediated crosstalk and cellular microenvironment conducive to cholangiocarcinoma, Neoplasia, Arunsan P, Chaidee A, Cochran CJ, …, Ittiprasert W
- Hemozoin from the liver fluke, Opisthorchis felineus, modulates dendritic cell responses in bronchial asthma patients, Frontiers in Veterinary Science, Saltykova IV, Ittiprasert W, Nevskaya KV et al., 2019
- Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni, Elife, Ittiprasert W et al., 2019
- Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity, Elife, Arunsan P#, Ittiprasert W#, Smout MJ
- Et al., 2019
- Identification and characterization of functional expressed sequence tags-derived simple sequence repeat (eSSR) markers for genetic linkage mapping of Schistosoma mansoni juvenile resistance and susceptibility loci in Biomphalaria glabrata, International Journal for Parasitology, Ittiprasert W et al., 2013
- Impairment of CD4+CD25+ regulatory T cells in C4-deficient mice, Asian Pacific Journal of Allergy and Immunology, Leaungwutiwong P, Ittiprasert W, Saikhun K et al., 2011
- Reversing the resistance phenotype of the Biomphalaria glabrata snail host Schistosoma mansoni infection by temperature modulation, PLOS Pathogens, Ittiprasert W and Knight M, 2012
- Identification of immediate response genes dominantly expressed in juvenile resistant and susceptible Biomphalaria glabrata snails upon exposure to Schistosoma mansoni, Molecular and Biochemical Parasitology, Ittiprasert W et al., 2010
- Complete deficiencies of complement C4A/C4B including 2-bp insertion in codon 1213 are genetic risk factors of systemic lupus erythematosus in Thai populations, Journal of Autoimmunity, Ittiprasert W et al., 2005
- Differential diagnosis of schistosomiasis mekongi and trichinellosis in human, Parasitology International, Ittiprasert W et al., 2000
Leadership Experience
- Class teaching 04/2018-present
- Academic/Scientific editor for PLOS journals 03/2019-present
- Scientific reviewers for several journals 02/2016-present
- Leader for molecular biology reagent manufacturing at BRI 04/2011-11/2015
Awards
- Best Research Award, New Perspective in Biology&Infection Conference 09/2023
- Excellent Research Award, National Research Council of Thailand 06/2023
Timeline
Research Associate Faculty
George Washington University
01.2017 - Current
Research Assistant Faculty
George Washington University
12.2015 - 12.2016
Research Associate
Biomedical Research Institute (BRI)
04.2011 - 11.2015
Postdoctoral Fellow
Biomedical Research Institute (BRI)
01.2006 - 03.2011
Scientist
Mahidol University
03.2002 - 11.2005
Doctor of Philosophy - Tropical Medicine&Hygiene, major Immunology
Mahidol University
Bachelor of Science - Medical Technology
Mahidol University
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