Stearne Briem
Test Engineer - Metabolomics
Somerville,MA
Summary
Diversely trained Analytical Scientist demonstrating superior quality assurance and accuracy in recording results. Astute chemist with 5+ years of industry experience with analytical techniques, including LCMS, HPLC/uHPLC, and IC applications. Ready to join and grow within a new organization collaborating with other professionals in a laboratory environment.
Overview
6
6
years of professional experience
4
4
years of post-secondary education
Work History
Test Engineer II
Ginkgo Bioworks
04.2021 - Current
- Leads the development, validation, and deployment of uHPLC-UV/RID/SEC/DAD, HPLC-MS (QQQ), and IC targeted metabolomic methods to support the foundry and fermentation teams within Ginkgo
- Provides technical expertise to program teams related to liquid chromatography applications in order to develop accurate high-throughput instrumental analysis of bacterial strain genetic engineering projects
- Writes Standard Operating Procedure documentation on developed methods to transfer to Operators for service execution
- Mentors and trains junior Test Engineers on liquid chromatography applications and SOP generation
- Applies DOE approaches to refine and improve sample extraction and assay methods related to LC applications
- Collaborates with software engineers to ensure that all generated data is captured, stored, and analyzed in Ginkgo's LIMS and internal software resources.
Associate Biochemistry Scientist
J.R. Simplot Company, Plant Sciences
Boise, ID
02.2020 - 04.2021
- Study coordinator for proteomic and metabolomic characterization projects of strawberry and potato genetic engineering strategies
- Operator of Agilent GCMS (QQQ), Thermo Fisher HPLC-MS (QQQ and Qexactive Orbitrap), FTIR, IC, and Dumas instrumentation; new method development and optimization for target analytes
- Member of the Molecular and Cellular Biology research team
- Characterized target proteins for new potato varieties based on enzyme activity and protein abundance through MS
- Developed LCMS (Qexactive) methods for target analyte quantification for strawberry and potato backgrounds
- Provides metabolomics and proteomic support for regulatory submissions for deregulation
- Provides expertise relating the laser diffraction particle size analysis
Chemist I
J.R. Simplot Company, Food Group
Boise, ID
05.2018 - 02.2020
- Maintained operations within the Technical Services Chemistry laboratory contributing to Simplot's Quality, Food Safety and Regulatory compliance programs
- Managed the Laboratory Information Management System (LIMS) for data analysis, query, and analytical reporting
- Built interorganizational relationships between various company analytical laboratories
- Conducted research and validation of new technologies to implement in the Tech Center and/or plant labs to monitor wastewater and process streams
- Operated HPLC-MS, HPLC, and Nitrogen Dumas Instrumentation for process streams and wastewater analysis
- Supported Quality, Operations, R&D and Regulatory with consulting related to food chemistry
Research Technician II
J.R. Simplot Company
Boise, ID
08.2017 - 04.2018
- Scheduled, prepared, set-up, executed, and facilitated consumer & sensory taste tests
- This includes receiving and tracking all incoming samples and preparing reference and test samples
- Operated SPME-GC-MS instrumentation for untargeted analysis of volatile compounds linked for flavor profiling
- At test completion, reports data in MS Excel spreadsheets and analyzed data
- Completed large data set analyses for identification of trends and/or correlation Supported and/or lead the development of protocols for sensory method development and analysis.
Student Research Assistant
The College of Idaho, Dr, John Thurston Lab
Caldwell, ID
09.2015 - 03.2017
- Assisted a material chemistry research project involving synthesis of photoactive-carbon nitride hydrogels for antimicrobial applications Performed routine laboratory duties including reagent preparation and cleaning
- Compiled FTIR data and Presented research at student research conferences
Education
Bachelor of Science - Chemistry, Health Science/Political Economy/
The College of Idaho
08.2013 - 05.2017
Skills
Xcalibur
Chromeleon
HPLC-MS Instrumentation Operation
IC Instrumentation Operation
GC/GCMS Instrumentation Operation
LC/IC Method Development
LCMS Method Development
Spanish
Project Management
Technical Writing
Timeline
Test Engineer II
Ginkgo Bioworks
04.2021 - Current
Associate Biochemistry Scientist
J.R. Simplot Company, Plant Sciences
02.2020 - 04.2021
Chemist I
J.R. Simplot Company, Food Group
05.2018 - 02.2020
Research Technician II
J.R. Simplot Company
08.2017 - 04.2018
Student Research Assistant
The College of Idaho, Dr, John Thurston Lab
09.2015 - 03.2017
Bachelor of Science - Chemistry, Health Science/Political Economy/
The College of Idaho
08.2013 - 05.2017
Hobbies
- Regional US Soccer Referee
- Mt. Biking
- Skiing
- Piano
Hobbies
- Regional US Soccer Referee
- Mt. Biking
- Skiing
- Piano