Amanda Peterson

• Executing, analyzing, and troubleshooting in vitro experiments to characterize viral vector designs and screening.
• Molecular biology techniques (Cloning, Western blotting, DNA/RNA extractions, PCR, RT-PCR, quantitative PCR, fluorescence microscopy, imaging,.)
• Working collaboratively with other department team members and in various therapeutic focus areas.
• Participation in team meetings and preparation of regular presentations for departmental meetings.
• Enhanced research efficiency by streamlining data collection and analysis processes.
• Conducted comprehensive literature reviews to inform study designs and methodology decisions.
• Wrote research papers, reports, reviews, and summaries regarding gene therapy.
• Generated data models and performed analysis to produce reports outlining results.
• Worked as member of integrated project team in highly collaborative work environment.

● Perform RNA purification, Western Blotting, PCR, MSD, Toxicity Assays using ENVISION, ELISA,
Enzyme-Based Assays, BCA, Bradford, DNA Purification
● Drive research projects with little to no supervision.
● Create research questions, hypotheses, and guidelines for experiments related to protein/cells of
interest.
● Perform mammalian tissue culture / Maintenance of various cell lines
● Use countess cell counter
● Present data at group meetings
● Usage of GraphPad PRISM
● Read and research current publications relevant to ongoing research
● Maintain detailed laboratory notebooks
● Identification of New Chemical Entities (NCEs)
● Screen compounds and support structure activity relationship (SAR)

• Perform immunoassays on the following platforms: ELISA, Mesoscale ECL,
alphaLISA, MSD, Luminex
• Perform BCA, Bradford, and other bioanalytical/Immunohistochemistry
experiments.
• Utilize ligand-binding bioanalytical methods to measure biotherapeutics (PK)
• Compliance with Good Laboratory Practice (GLP).
• Apply scientific theory and techniques to research problems
• Characterization of compounds such as ADME studies, protein binding.
• Aseptic Mammalian cell culture, and maintenance.
• Use of cell counter for cell viability studies

*Short experience at GSK due to death in family, needed to move back to NJ
• Design experiments to support early phase drug discovery or development.
• Preform analysis of finished API’s or Intermediates using XRPD, TGA, DSC, TGA-IR,
Raman, Optical Microscopy, Polarized Light Microscopy
• Utilize electronic notebooks
• Determine the structure, composition, and nature of drug substances.
• Perform data interpretation and review
• Comply with regulatory and data integrity requirements, including cGMP and GSK
policies and procedures. • Work independently to optimize analytical methods.
• Work both independently and within a group matrix environment.
• Keeping up to date with scientific literature and research.

• Perform and maintain bacterial cultures & identify microbial identity via Gram Staining
and VITEK.
• Understand and practice of aseptic technique while working in Work in BSL I&II
Containment hoods.
• Perform raw material, intermediate, final product, stability and water testing via
bioburden/endotoxin testing.
• Full understanding of cGMP laboratory requirements, GDP, SOPs, ICH guidelines, USP
requirements and FDA guidance.
• Monitor all physiological and morphological characteristics of bacterial, yeast or mold
colonies present on media plates (RODAC, TSA, R2A, etc.).
• Perform data input, reviews, and analyses.
• Ensures deadlines are met for product development schedules or releases.

• Compound capsules (#3, #1, #0, #00), creams, suspensions, troches, nasal sprays,
topical ointments, drops, and suppositories.
• Work with FDA controlled substances, hormones, and hazardous/nonhazardous chemicals.
• Utilize full USP 800 and 795 guidelines and gowning procedures.
• Understand and perform pharmaceutical calculations to obtain correct medication dosage.
• Adhere to all state and federal pharmacy regulations

• Participate in early stage physicochemical characterization if API’s or Intermediates by use
of HPLC, KF, Coulometric KF, PLM, DVS, PSD, TGA, DSC, XRPD and NMR.
• Tailor API or intermediate material to balance drug processability and drug
performance (solubility, dissolution, permeability, etc.)
• Executes non-routine experiments on a basis of literature analyses.
• Assumes responsibility for the design and technical accuracy of work.
• Perform routine assays, per client’s request with little to no supervision.
• Present and create summarized data/project updates to clients on a weekly or
biweekly basis.

• Maintain accurate lab notebooks and complete all related analytical
reports, write summaries, and keep proper documentation