Andressa Reginato
Bronx,NY
Summary
I have experience working as a researcher and teacher (classes and/or training juniors). I consider myself as a hardworking and passionate job seeker with strong organizational and team communication skills. I'm always ready to help team achieve the best for promoting and recovering human health using my laboratory/ researcher skills.
Overview
11
11
years of professional experience
Work History
Flow Cytometry Technologist
Montefiore Medical Center Moses Division
01.2023 - Current
- Mentor of junior team members to enhance their technical skills and improve overall team productivity.
- Coordination of inventory supplies and equipment to maximize team productivity.
- Preparation of reagents and solutions used in staining and flow cytometry analysis of samples
- Assistance in training staff, residents, fellows and/or other trainees
- Analyzes in flow cytometric software and reports results of immunophenotyping and CD34+ stem cell enumeration to Stem Cell Laboratory personnel.
Post-doctoral researcher
Albert Einstein College of Medicine
01.2021 - 01.2023
- I worked with 2 projects during the period. One of them was related to the investigation of the molecular mechanisms involved in the benefits of twice a night feeding in mice. I used flow cytometry to assess immune cells in this model. The second project was the investigation of autophagy and immune cells in peripheral tissues of PS19 mice, a genetic model of Tau accumulation.
- I used flow cytometry as major technique to assess immune cells from stromal vascular cells of adipose tissue.
Post-doctoral position
State University of Rio de Janeiro
01.2017 - 02.2021
In this period of post doctoral research I worked with hypothalamic tissue from fetuses that were generated in an obese environment. I also conducted a systematic review on the role of fatty acids in ceramides pathways and their influence on hypothalamic regulation of energy balance. Those records can be found on Pubmed.
Nutrition professor
Einstein Faculty (Faculdades Integradas Einstein de Limeira)
01.2019 - 01.2020
- Professor of Human Nutrition and Etics.
Master and PhD researcher
University of Campinas (Unicamp)
01.2013 - 01.2019
- Researcher working with the effects of saturated fat acids on autophagy in neuronal cells and the hypothalamus.
Education
Phd in Nutrition, Sports and Metabolism -
University of Campinas
Limeira, Brazil01.2015
Visiting PhD student -
Physiology Department, University of Toronto
Toronto, Canada01.2015
B.S. in Nutrition -
University of Campinas
Limeira, Brazil01.2012
Skills
- In vivo: experience with rodent (mouse) model involving animal handling, establishing and maintaining transgenic mouse colonies, breeding, weaning, genotyping, health monitoring, anesthesia, injections, gavages, perfusions, dissections, metabolic tests, stereotaxic surgery
- In vitro: aseptic techniques, establishment and maintenance of several cell lines, including hepatocytes, neurons and microglia Transfection, viral transduction, cryopreservation, critical experiments involving chemical compounds
- Protein Assays: protein extraction, Western Blot, Immunoprecipitation, ELISA
- Molecular analysis: DNA and RNA extraction and purification from cultured cells and animal tissues, cDNA preparation, PCR, RT-qPCR
- Staining and image analysis: immunofluorescence, immunohistochemistry
- Flow Cytometry (BD Accuri and Cytek-Aurora) working with autophagy detection and spectral cytometry assessing immune cells from bone marrow and stromal vascular cells from adipose tissue (B, T, ILC2, macrophages and progenitors cells)
- Computer Skills: Office; GraphPad Prism; ImageJ; Flow-Jo
Personal Information
- I am a researcher from Brazil, who is passionate about science in general, specially studying nutrition, autophagy, and metabolism. I am particularly interested in working with the technique of flow cytometry, that I have used to assess autophagy in neurons and am currently using to assess immune cells from stromal vascular fraction. I enjoy working collaboratively with other researchers in the lab and I also have a passion for educating other scientists, students, and the public about issues in science and nutrition.
- Title: NUTRITIONIST, PHD
- Date of Birth: 03/07/90
- Nationality: Brazilian
Publications
- Saturated Fatty Acids Modulate Autophagy's Proteins in the Hypothalamus, Plos One, 10, e0119850, 2015, PORTOVEDO, M., IGNACIO-SOUZA, L M., BOMBASSARO, B, COOPE, A, REGINATO, A, RAZOLLI, DS., TORSONI, MA., TORSONI, AS., LEAL, R.F., VELLOSO, LA., MILANSKI, M., AGUILA, MB.
- Lipid excess affects chaperone-mediated autophagy in hypothalamus, BIOCHIMIE, 176, 110116, 2020, PORTOVEDO, M., REGINATO, A., MIYAMOTO, J.É., SIMINO, L.A., HAKIM, M.P., CAMPANA, M., LEAL, R.F., IGNÁCIO-SOUZA, L.M., TORSONI, M.A., MAGNAN, C., LE STUNFF, H., TORSONI, A.S., MILANSKI, M.
- Acute effects of fatty acids on autophagy in NPY neurones, J Neuroendocrinol, 32, 10, e12900, 2020, REGINATO A, SIQUEIRA BP, MIYAMOTO JÉ, PORTOVEDO M, COSTA SO, DE FANTE T, RODRIGUES HG, IGNÁCIO-SOUZA LM, TORSONI MA, TORSONI AS, LE STUNFF H, BELSHAM DD, MILANSKI M
- The Role of Fatty Acids in Ceramide Pathways and Their Influence on Hypothalamic Regulation of Energy Balance: A Systematic Review, Int J Mol Sci, 22, 10, 5357, 2021, REGINATO A, VERAS ACC, BAQUEIRO MDN, PANZARIN C, SIQUEIRA BP, MILANSKI M, LISBOA PC, TORSONI AS
References
- Dr. Marciane Milanski, Professor, marcia31@unicamp.br
- Dr. Hosana Gomes Rodrigues, Professor, hosana.rodrigues@fca.unicamp.br
- Dr. Jinghang Zhang, Operator Director, jinghang.zhang@einsteinmed.edu
- Ms. Ming Liu, Operator, ming.liu@einsteinmed.edu
- Ms. Aodengtuya Fnu, Operator, fnu.aodengtuya@einsteinmed.edu
Timeline
Flow Cytometry Technologist
Montefiore Medical Center Moses Division
01.2023 - Current
Post-doctoral researcher
Albert Einstein College of Medicine
01.2021 - 01.2023
Nutrition professor
Einstein Faculty (Faculdades Integradas Einstein de Limeira)
01.2019 - 01.2020
Post-doctoral position
State University of Rio de Janeiro
01.2017 - 02.2021
Master and PhD researcher
University of Campinas (Unicamp)
01.2013 - 01.2019
Phd in Nutrition, Sports and Metabolism -
University of Campinas
Visiting PhD student -
Physiology Department, University of Toronto
B.S. in Nutrition -
University of Campinas