Di He
New York,NY
Summary
- Current Post Doc and PhD Biology graduate
- Excellent technical skills in molecular and cell biology with two first author papers published
- Sound understanding of RNA-seq and single cell RNA-seq analysis, animal models, and pathway analysis
- Strong cancer organoids and mouse models establishing skills
- Keen to apply technical and project management skills to an industry role ·
Overview
3
3
years of professional experience
Work History
Research Fellow
Memorial Sloan Kettering
New York, NY
11.2019 - Current
Highlighted a link between SMARCA4 loss-of-function and increased metastasis in lung adenocarcinomas, indicating that ROR2/p-Gsk3b pathway is directly regulated by SMARCA4 loss-of-function and contributes to tumor aggressiveness.
Education
Ph.D. - Biology
Tongji University
Shanghai06.2019
Bachelor of Science - Biology
University of Science And Technology Beijing
Beijing06.2014
Skills
- Molecular biology skills such as gene editing using shRNA and CRISPR, cloning, Western Blot, real-time qPCR, CHIP-seq, Co-IP, immunostaining;
- RNA-seq analysis and single cell RNA-seq analysis using R packages DEseq2 and seurat, with down stream GSEA, KEGG and GO analysis
- Tumor model establishment including in vtiro cell line and organoid models and animal injection/xenograft models through subcutaneous injection, tail vein injection and intra-trachea injection
Published papers
1. He, D., Wang, D., Lu, P. et al. Single-cell RNA sequencing reveals heterogeneous tumor and immune cell populations in early-stage lung adenocarcinomas harboring EGFR mutations. Oncogene 40, 355–368 (2021).
2. He, D., Zhang, J., Wu, W., Yi, N., He, W., Lu, P., Li, B., Yang, N., Wang, D., Xue, Z., Zhang, P., Fan, G., and Zhu, X. (2019) A novel immunodeficient rat model supports human lung cancer xenografts. Faseb j 33, 140-150
3. Liu. Y, Chudgar. N, Mastrogiacomo. B, He. D et al. Targeting c-fos to suppress metastasis in BRMS1v2 germline mutantlung adenocarcinoma. Science Translational Medcine. Accepted (2022).
4. He D., Zhu X., Xu S., Xue Z. and Fan G. (2015) Recent advancement in the generation of genetic modified mammalian animal models via CRISPR/Cas9. Review. Chinese Journal of Cell Biology, 37(9): 1295–1301
5. Xu, S., Zhu, X., Li, H., Hu, Y., Zhou, J., He, D., Feng, Y., Lu, L., Du, G., Hu, Y., Liu, T., Wang, Z., Ding, G., Chen, J., Gao, S., Wu, F., Xue, Z., Li, Y., and Fan, G. (2016) The 14th Ile residue is essential for Leptin function in regulating energy homeostasis in rat. Scientific reports 6, 28508
6. Yang, J., Yi, N., Zhang, J., He, W., He, D., Wu, W., Xu, S., Li, F., Fan, G., Zhu, X., Xue, Z., and Zhou, W. (2018) Generation and characterization of a hypothyroidism rat model with truncated thyroid stimulating hormone receptor. Scientific reports 8, 4004
Timeline
Research Fellow
Memorial Sloan Kettering
11.2019 - Current
Ph.D. - Biology
Tongji University
Bachelor of Science - Biology
University of Science And Technology Beijing
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