Emmanuel Opoku

Quality-focused Laboratory worker of six years of experience in a laboratory setting. Working at Cleveland Clinic and Case Western to perform various experiments and worked on different projects under minimal supervision. Critical thinking Research Technician demonstrating superior data gathering and analysis skills. Astute professional knowledgeable in laboratory terminology, procedures and statistical methods. Highly trained individual with expertise performing experimental testing. Multitasking Research Technician knowledgeable in preparation of needed reagents and buffers. Hope to gain admission into the Medical Physiology program to enhance preparation towards medical school.

• Experienced in many laboratory skills and equipment usage such as:
• The use of qPCR to quantify viral particles.
• Usage of spectrophotometer to determine DNA concentration.
• Performed Bacteria transformation and Colony PCR
• Usage of LC-MS and HPLC-MS to detect and identify the particular masses of chemicals in the mix of other chemicals such as natural products from natural product extracts and pure substances from a mixture of chemical intermediates.
• Performed transfection of virus into Jurkats T cells and splitting of cells.
• And experienced in other laboratory procedures such as RT-Assay, mini prep, Maxi prep, Midi Prep, extraction of RNA from virus, gel electrophoresis, sonication, ELISA, western blot, and data analysis.
• Performing Titrations
• Excellent hand-eye coordination and vision to inspect small parts

• President of NAACP Youth Council, Topeka KS
• Captain of Intramural soccer team

• Experience in assisting in establishing and scheduling of various procedures and analysis.
• Experience with cadavers/deceased persons
• Able to assist in ordering laboratory supplies and equipment
• Able to interact effectively and positively with other Co-Workers
• Experience working with animals
• Familiar with commonly used concepts, practices and procedures within the field of Molecular Biology and/or Biochemistry.
• Strong organizational skills and attention to scientific detail.
• Ability to work independently and under pressure, multi-task, and be a team player
• Professional and effective verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues, supervisors, and customers face to face.
• Ability to rely on instructions and pre-established guidelines to perform the functions of the job.
• Ability to follow-through on assigned projects and provide timely follow-up as needed.
• Ability to problem-solve effectively; must demonstrate good analytical skills, sound judgement and good decision-making.
• Ability to effectively operate basic laboratory equipment.
• Working knowledge of Microsoft Office (Word, Excel, PowerPoint) and database applications. Ability to use a scientific graphics package (such as MATLAB, Origin, or Sigma plot), is strongly preferred.
• Must demonstrate willingness to learn new techniques, procedures, processes and computer programs as needed.
• Ability to meet consistent attendance.
• Well organized and able to self-direct daily activities.
• Ability to create and maintain records and technical data associated with job responsibilities

• Decreased FAM13B expression increases atrial fibrillation susceptibility by regulating sodium current and calcium handling. https://www.jacc.org/doi/10.1016/j.jacbts.2023.05.009
• Fine Mapping of the Mouse Ath28 Locus Yields Three Atherosclerosis Modifying Sub-Regions., https://www.mdpi.com/2073-4425/13/1/70
• Gasdermin D Mediates Inflammation-Induced Defects in Reverse Cholesterol Transport and Promotes Atherosclerosis., https://www.frontiersin.org/articles/10.3389/fcell.2021.715211/full
• Uptake of high-density lipoprotein by scavenger receptor class B type 1 is associated with prostate cancer proliferation and tumor progression in mice., https://pubmed.ncbi.nlm.nih.gov/32358065/
• Soat1 mediates the mouse strain effects on cholesterol loading-induced endoplasmic reticulum stress and CHOP expression in macrophages., https://pubmed.ncbi.nlm.nih.gov/33031913/
• Oxidant resistant human apolipoprotein A-I functions similarly to the unmodified human isoform in delaying atherosclerosis progression and promoting atherosclerosis regression in hyperlipidemic mice., https://pubmed.ncbi.nlm.nih.gov/35120132/

• Dr. Cynthia A Traughber, Post-Doctoral Fellow, Cleveland Clinic/Cleveland State University, 615-445-3376, c.traughber@csuohio.edu
• Dr. Kailash Gulshan, Principal Investigator, Cleveland Clinic/Cleveland State, 319-631-8232, k.gulshan@csuohio.edu