Isabella De La Concha Escobedo
Summary
I am detail-oriented individual who is eager to join a research team and make meaningful contributions to science, particularly in the fields of Molecular Biology and Immunology. I offer a range of skills, including adaptability and mindfulness, which are important for successful teamwork.
Overview
3
3
years of professional experience
Work History
Summer Internship
Sherwood Lab, Brigham and Women's Hospital
04.2025 - 08.2025
- I worked with CRISPR-Cas9 genetic manipulation, including molecular cloning, lentiviral transduction, and culturing various cell lines such as HepG2, HEK293, and U2OS. My primary responsibilities involved performing multiple gene knockouts (KO) using lentiviral transduction, conducting DNA extraction, PCR amplification, and PCR product purification, as well as analyzing DNA sequences and chromatograms using SnapGene. Two of the main projects I contributed to included establishing a TASOR KO rtTA BFP U2OS cell lines for future experiments, and cloning a dPAM-dCas9-P2A-mCherry plasmid for future lentivirus production and infection.
- Full-Time
CD133: An Immunotherapeutic Target Against Hepatocellular Carcinoma
Massachusetts College Of Pharmacy And Health Sciences
01.2025 - 12.2025
- This independent project aimed to increase the expression of CD133, a cell-surface glycoprotein that has the potential to become a molecular biomarker for hepatocellular carcinoma, and therefore, a more viable target for immunotherapeutic options such as CAR T-cell therapy. HepG2 cells were stimulated with IL-6 in order to increase the expression of CD133. This cytokine was chosen as the stimulant due to its role in the JAK-STAT pathway. This project was an award winner of the 2025 MCPHS Center for Research and Discovery Conference.
- Part-Time
Testing the Anti-Inflammatory Property of Chalcones
Massachusetts College Of Pharmacy And Health Sciences
09.2024 - 12.2025
- This independent project, funded by an MCPHS mini grant, aimed to develop anti-inflammatory chalcone derivatives that reduce cytokine production in stimulated cell cultures. Chalcones were synthesized via aldol condensation reactions using benzaldehydes (4-hydroxy-3-nitrobenzaldehyde and 4-(1H-imidazol-1-yl)benzaldehyde) and various acetophenones. The derivatives were tested in intestinal epithelial cells (HT-29) stimulated with LPS to induce cytokine production (IL8).
- Part-Time
Microbiology and Molecular Biology Peer Tutor
MCPHS
09.2024 - 12.2025
- I support students taking Microbiology and Molecular Biology courses bu helping them build a stronger understanding of core concepts such as cell structure, genetic expression, and microbial interactions. I encourage independent learning, critical thinking, and academic integrity by breaking down complex topics, drawing concept maps, and adapting to the student's learning style.
- Part-Time
International Student Ambassador
Massachusetts College Of Pharmacy And Health Sciences
11.2022 - 12.2025
- As an International Student Ambassador (ISA), I play a crucial role at MCPHS University by serving as representatives for the international community. My primary responsibilities include helping international students acclimate to campus life, assisting with orientation programs and academic resources, and providing information on immigration regulations and assisting with related paperwork.
- Part-Time
Education
Bachelor of Science - Medical And Molecular Biology
Massachusetts College of Pharmacy And Health Sciences
Boston12-2025
Minor - Chemistry
Massachusetts College of Pharmacy And Health Sciences
Boston12-2025
International Baccalaureate (IB) -
Escola Americana De Campinas
São Paulo, Brazil05-2022
American High School Curriculum -
Escola Americana De Campinas
São Paulo, Brazil05-2022
Skills
- Cell Culturing (HepG2, HEK293, U2OS), Stimulation Assays, Lentivirus Production and Transduction, CRISPR/Cas9, Cloning, Molecular Biology Techniques (DNA extraction, PCR, Gel Electrophoresis, etc)
- NMR, Spectroscopy, Gravimetric Analysis, Quality Assurance, Quality Control
- Microsoft Software (Word, Excel, PowerPoint, etc), SnapGene
- Teamwork, Leadership, Communication, Critical Analysis, Organization, Critical Thinking, Creativity, Problem-Solving, Troubleshooting
Languages
English
Native or Bilingual
Spanish
Native or Bilingual
Portuguese
Full Professional
Timeline
Summer Internship
Sherwood Lab, Brigham and Women's Hospital
04.2025 - 08.2025
CD133: An Immunotherapeutic Target Against Hepatocellular Carcinoma
Massachusetts College Of Pharmacy And Health Sciences
01.2025 - 12.2025
Testing the Anti-Inflammatory Property of Chalcones
Massachusetts College Of Pharmacy And Health Sciences
09.2024 - 12.2025
Microbiology and Molecular Biology Peer Tutor
MCPHS
09.2024 - 12.2025
International Student Ambassador
Massachusetts College Of Pharmacy And Health Sciences
11.2022 - 12.2025
Bachelor of Science - Medical And Molecular Biology
Massachusetts College of Pharmacy And Health Sciences
Minor - Chemistry
Massachusetts College of Pharmacy And Health Sciences
International Baccalaureate (IB) -
Escola Americana De Campinas
American High School Curriculum -
Escola Americana De Campinas
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