Jiaqi Wu
Los Angeles,CA
Summary
Innovative Researcher adept at developing probing questions, designing experiments and generating meaningful results. Experienced at identifying and using new technologies and resources. Works effectively and collaboratively with teams and individuals.
Overview
4
4
years of professional experience
Work History
Research Associate
Cedars Sinai Comprehensive Cancer Center
07.2023 - Current
- Cedars-Sinai using cutting edge -omics technologies, a wide range of innovative animal cancer models, and organoids to elucidate the biological mechanisms of sex differences in bladder cancer; we recently published several works that pioneer new concepts on sex as a biological variable in cancers
- Main tasks:
- Carry out basic molecular techniques, 3D-organoid culture and maintain, and live animal surgery techniques
- Cultivate cell lines from human and animal tissues, create bacterial stocks, and revive bacteria and cell line as needed
- Carry out sequencing library preps, Conduct PCR and qRT-PCR assays, perform ELISAs,IF/IHC staining, master additional skills such as flow cytometry, CUT&RUN, FACS sorting, sample preparation for single-cell and single-nuclei sequencing, andadvanced microscopy.
- lab management and lab supply purchasing.
Project Researcher
USC
01.2022 - 06.2023
- Young-Kwon Hong Laboratory, Norris Comprehensive Cancer Center at Keck school of Medicine of USC
- Establishing the CD200 KO through CRISPR-Cas9 and crossbreeding with Prox1-GFP lymphatic-specific fluorescent reporter mouse line
- Visualization of the lymphatics in tissues and lymph nodes comparing differences of CD200 KO vs
- WT under fluorescence microscopy
- Creating ear skin inflammatory mice model of CD200 KO vs WT and figured out CD200 serves as the immune inhibitor in inflammation regulation and CD200 KO mice showed abnormal enlarged lymph nodes
- Determine immune cells comprise CD200 KO abnormal lymph nodes vs
- WT mice using cryosection and immunofluorescence staining and imaging under fluorescence microscopy
- CrossBreeding CD200 KO mice with Kaposi sarcoma (KS) inducible mouse model, showing CD200 KO mice tumor formation is slower than WT Kaposi sarcoma (KS) mice, collected tumor from mice and to do decalcification, cryosection, H&E staining
- Cell culture and Immunofluorescence staining with CD200 Ab and CD200R1 Ab to confirm CD200 expression in LECs
Project Researcher
Gut Microbiota and Host, Hui Wang Laboratory
08.2019 - 06.2021
- School of Life Science of Nanjing Agricultural University
- Verifying human gut microbiota can secrete DING protein through fecal microbiota transplantation into mice and Western Blot
- Isolating a non-acid-producing fungus with inhibitory effect on the growth of Vibrio cholerae from the soil(S10-1) and identifying the strains was Trichoderma reesei by microscope observation of biological morphology, 18S rRNA sequencing and drawing Phylogenetic tree by NCBI-Blast and MEGA 8.0
- Exploring the inhibiting molecular mechanism of Vibrio.cholerae by constructing a transposon gene library; through arbitrary PCR, electrophoresis gel running, and gel recycling; comparing sequencing results in Clone Manager obtaining key genes were vprB and dbpA, and determining the regulation mechanism of colonization and the antibacterial effect of the supernatant by genetic knockout and replenishing-strains construction
- Screening gut fungi having similar inhibitory effect to pathogenic bacteria including Vibrio cholerae, klebsiella.pneumoniae, and Escherichia.coli
Education
Master of biochemistry and molecular - biology
University of Southern California, Keck School of Medicine
Los Angeles, CA05.2023
Bachelor of Science - Biotechnology
Nanjing Agricultural University, School of Life Science
Nanjing, China06.2021
Skills
- Laboratory: DNA, RNA, Protein extraction, pipettes, viral vector and bacteria and fungi culture and preservation, mammalian cell culture and preservation, 3D-organoids culture and preservation, Flow Cytometry, RT-PCR, qPCR, CUT &TAG, CUT&RUN, FACS, mice perfusion, intraperitoneal injection, tail injection, Construction of recombinant plasmids
- Computer: Microsoft Office, R, Prism, Fiji(Image J), SPSS, Clone Manager, Photoshop, Leica,QuantStudio, Sanger Sequencing,Veriti Thermo Cycler
Languages
English
Chinese (Mandarin)
Chinese (Cantonese)
Timeline
Research Associate
Cedars Sinai Comprehensive Cancer Center
07.2023 - Current
Project Researcher
USC
01.2022 - 06.2023
Project Researcher
Gut Microbiota and Host, Hui Wang Laboratory
08.2019 - 06.2021
Master of biochemistry and molecular - biology
University of Southern California, Keck School of Medicine
Bachelor of Science - Biotechnology
Nanjing Agricultural University, School of Life Science
Similar Profiles
Celeste CampbellCeleste Campbell
Oncology RN at Cedars Sinai Comprehensive Cancer CenterOncology RN at Cedars Sinai Comprehensive Cancer Center
Chelsea BergChelsea Berg
Clinical Nurse at Cedars Sinai Comprehensive Cancer CenterClinical Nurse at Cedars Sinai Comprehensive Cancer Center
Kim CopelandKim Copeland
Sr. Administrative Assistant at Cedars-Sinai Medical CenterSr. Administrative Assistant at Cedars-Sinai Medical Center