Summary
Overview
Work History
Education
Skills
Publications
Presentations
Timeline
Generic

JIMIANE L. ASHE

Marysville,WA

Summary

Talented professional considered knowledgeable leader and dedicated problem solver. Brings 30 years of valuable expertise to forward company objectives. Attentive to detail with experience in coordinating research projects, programs, improvements, and education.

Overview

26
26
years of professional experience

Work History

Project Manager II, Research Manager

Seattle Children’s Hospital General Surgery Division
05.2022 - Current
  • Experimental design, ethics approval, data collection, statistics, manuscript preparation for 60+ projects under 14 surgical faculty members.
  • Accomplished multiple tasks within established timeframes.

Adjunct Assistant Professor

City University Of New York Baruch & Brooklyn
08.2021 - 12.2021
  • Instructor for undergraduate Fundamentals of Ecology recitation ENV1003 and laboratory ENV1004, General Biology lecture BIOL1001, and Fundamentals of Biology recitation BIO1015 and laboratory BIO1016.
  • Enhanced student comprehension by employing diverse teaching methodologies and incorporating real-world examples.

Non-Teaching Adjunct

City University Of New York Open Educational Resources Initiative (NSF Award #1827035)
12.2018 - 09.2019
  • Authored, produced and edited 15 online educational tutorial and resource videos for undergraduate microbiology laboratory BIOL3004.
  • Demonstrated software use and data analyses.
  • Collected environmental samples including soil, sediments, water, plants, feces and invertebrate organisms for analyses.
  • Metagenomic DNA extraction for all environmental sample types.

Graduate Student Instructor

City University Of New York Brooklyn College
08.2013 - 05.2019
  • Adjunct Instructor for undergraduate general biology laboratory BIOL1001 and BIOL1002.
  • Supervised and maintained the genetic and marine aquatic laboratory facilities including equipment maintenance and animal care.
  • Designed, advised and oversaw research projects for 9 undergraduate students.
  • Created lesson materials, visuals, and digital presentations to supplement lesson plans.
  • Streamlined grading processes for efficiency, providing timely feedback to students and promoting academic growth.
  • Scuba, beach seining, anesthetization, dissection and non-lethal tissue sampling of syngnathid species Hippocampus abdominalis (pot-bellied seahorse), Hippocampus erectus (lined seahorse) and Syngnathus fuscus (northern pipefish) in New Zealand and the United States (New York, Virginia, North Carolina and Florida).

Genetics Intern

City University Of New York Sackler Institute For Comparative Genomics, American Museum Of Natural History
09.2012 - 05.2013
  • Genomic DNA extraction from nucleated blood and tissues, PCR, Sanger sequencing, genotyping and data analyses.

Graduate Research Assistant

State University Of New York Stony Brook
08.2010 - 07.2011
  • Genomic DNA extraction, PCR, Sanger sequencing, genotyping and analysis for Chondrichthyes.
  • Utilized advanced statistical software to analyze large datasets, uncovering key insights for informed decision making.
  • Assisted in manuscript preparation, contributing to the publication of influential articles in peer-reviewed journals.
  • Undergraduate Teaching Assistant for General Chemistry laboratory CHE133 and Coastal Oceanography MAR333.
  • Annual Negaprion brevirostris (lemon shark) juvenile census in the lagoon nurseries of Bimini islands using 180-meter gill nets and non-lethal tissue sample collection for genetic analyses.
  • Long-line adult sharks surveys using gangeons on horizontal lines in 2-m water and on 600-m vertical lines. Acoustic tracking of juveniles in the nursery lagoon to study behavior and range.
  • Hook-and-line fishing and receiver retrieval for acoustic telemetry of Morone americana (white perch) in Long Island, New York.

Genetics Lab Technician

Seattle Aquarium
03.2002 - 04.2009
  • Genomic DNA extraction, PCR, Sanger sequencing, genotyping and analysis.
  • Larval Husbandry for multiple marine vertebrate and invertebrate species.
  • Community outreach presenter for Puget Sound marine invertebrates and Pacific Salmonids.
  • Behavioral observation and genetic analyses of non-lethal tissue samples for Hexanchus griseus (sixgill sharks).

Genomics Specialist II

Fred Hutchison Cancer Research Center Genomics Shared Resources
10.2005 - 04.2009
  • Genomic DNA extraction, PCR, Sanger sequencing, genotyping, Quantitative (Real-Time) PCR, Reverse Transcription PCR, microarray hybridization on Agilent and Roche NimbleGen Platforms.
  • Followed all company policies and procedures to deliver quality work.

Research Associate II

Xcyte Therapies, Inc. Research And Development Team
02.2004 - 08.2005
  • Cell line culture, genomic DNA extraction, PCR, Sanger sequencing, genotyping, Reverse Transcription PCR.

AmeriCorps*VISTA

Panhandle Health District #1
09.1998 - 10.1999
  • Curriculum development and secondary school instructor for adolescent pregnancy, HIV, and STI prevention.
  • Assisted laboratory development of more accurate genetic testing methods for STIs.
  • Self-motivated, with a strong sense of personal responsibility.

Education

Ph.D. - Ecology, Evolutionary Biology And Behavior

City University of New York Graduate Center
New York, NY
06.2021

Master of Science - Marine & Atmospheric Science

State University of New York
Stony Brook, NY
08.2011

Bachelor of Science - Cell & Molecular Biology

University of Washington
Seattle, WA
08.1998

Skills

  • Project planning, development and management
  • Publication and grant wrighting
  • Field research and sample collection including SCUBA Divemaster (PADI certified)
  • Computation, word processing and presentation software on Mac and PC platforms including Adobe and Microsoft software
  • Command-line statistical analyses in the R software environment
  • Illumina 16S microbiome next-generation sequencing and analysis in Qiime, R, and Microsoft Excel
  • DNA extraction, PCR and genetic sequencing, microsatellite detection, sequence and fragment analysis, and population genetics statistical analyses
  • Bayesian coalescent-base population analyses using command-line IMa2
  • Population level genetic analyses using Arlequin, DNAsp, GenAlEx, jModelTest, STRUCTURE, PHASE, and R
  • Species Distribution Modeling using QGIS, MaxEnt and R
  • Genetics-based systematics using Geneious, GeneMapper, PhyML, BEAST, Mesquite and FigTree
  • Annual Responsible Conduct of Research (RCR) interactive training and certification

Publications

  • Congenital Diaphragmatic Hernia and Hirschsprung’s Disease Comorbidities Case Report, Journal of Pediatric Surgery Case Reports, In Progress, Foster C, Ashe J, Rice-Townsend S, Stark R
  • Evaluation of Swallow Function in Patients with Congenital Diaphragmatic Hernia, In Progress, Kwon EG, Foster C, Ashe J, Ramaraj A, Stark R, Rice-Townsend S
  • Microbial-host associations in a nearshore marine vertebrate (Hippocampus abdominalis), In Progress, Ashe JL, Wilson AB
  • Isolating a mechanism for microbial-host local adaptation, In Progress, Ashe JL, Abassi A, Wilson AB
  • Comprehensive genus-wide screening of seahorse STRs identifies priority species for conservation assessment, Conservation Genetics Resources, Volume 3, 2, 221-230, 2021, Wilson AB, Ashe J, Padron M, Hamilton H
  • Navigating the southern seas with small fins: Genetic connectivity of seahorses (Hippocampus abdominalis) across the Tasman Sea, Journal of Biogeography, Volume 47, 1, 207-219, 2020, Ashe JL, Wilson AB
  • Local population structure and context-dependent isolation by distance in a large coastal shark, Marine Ecology Progress Series, Volume 520, 203-216, 2015, Ashe JL, Feldheim KA, Fields AT, Reyier EA, O’Connell MT, Brooks EJ, Skomal G, Gruber SH, Chapman DD
  • The behavioural and genetic mating system of the sand tiger shark, Carcharias taurus, an intrauterine cannibal, Biology Letters, Volume 9, 20130003, 2013, Chapman DD, Wintner SJ, Abercrombie DL, Ashe JL, Bernard AM, Shivji MS, Feldheim KA
  • Relatedness and polyandry of sixgill sharks, Hexanchus griseus, in an urban estuary, Conservation Genetics, Volume 12, 3, 679-690, 2010, Larson S, Christiansen J, Griffing D, Ashe J, Lowry D, Andrews K
  • Standardizing Analysis for the Quantification of V CDR3 T Cell Receptor Diversity, Journal of Immunological Methods, Volume 317, 1-2, 100-113, 2006, Long SA, Khalili J, Ashe J, Berenson R, Bonyhadi M

Presentations

  • Minimal operative complications with early repair on ECLS for Congenital Diaphragmatic Hernia: A single center experience, 2023 CHNC Annual Symposium, 2023, Denver, CO, Oral Poster
  • Longer extracorporeal life support and early Treprostinil to treat pulmonary hypertension in infants with severe congenital diaphragmatic hernia, UCSF CME: 16th International Conference Neonatal & Childhood Pulmonary Vascular Disease 2023, 2023, San Francisco, CA, Poster Presentation
  • Isolating a mechanism for microbial-host local adaptation and impacts on fitness, Virtual Evolution 2021, 2021, Oral Presentation
  • Isolating a mechanism for microbial-host local adaptation, Marine Evolution 2018, 2018, Strömstad, Sweden, Oral Presentation and Session Host
  • Microbial-Host Dynamics and the Evolution of Nearshore Diversity, Society for the Study of Evolution Annual Meeting, 2017, Portland, OR, Oral Presentation
  • Influence of Human-Associated Microbes on Protected Marine Species, Alumni Resources, NSF EAPSI 2016 Pre-Departure Orientation, 2016, Arlington, VA, Oral Presentation
  • Microbial-Host Dynamics and the Evolution of Nearshore Diversity, Aquatic Research and Environmental Assessment Center Seminar Series, Brooklyn College, 2015, Brooklyn, NY, Oral Presentation
  • Population genetic structure of lemon sharks in the western Atlantic, Aquatic Research and Environmental Assessment Center Seminar Series, Brooklyn College, 2014, Brooklyn, NY, Oral Presentation
  • Testing predictions of the “natal philopatry hypothesis” using lemon sharks (Negaprion brevirostris) in the Western Atlantic, Joint Meeting of Ichthyologists and Herpetologists/American Elasmobranch Society Conference, 2011, Minneapolis, MN, Oral Presentation
  • Is There Genetic Evidence of a Recent Population Bottleneck in White Sharks (Carcharodon carcharias) from the Northwest Atlantic?, Joint Meeting of Ichthyologists and Herpetologists/American Elasmobranch Society Conference, 2010, Providence, RI, Oral Presentation
  • Relatedness and polyandry of sixgill sharks, Hexanchus griseus, in an urban estuary, Joint Meeting of Ichthyologists and Herpetologists/American Elasmobranch Society Conference, 2009, Portland, OR, Oral Presentation
  • Ex vivo Expanded, Xcellerated T CellsTM, Produce a Diverse, Highly Proliferative Population of Functional T Cells, Immune Senescence and Homeostasis Keystone Conference, 2005, Keystone, CO, Poster Presentation

Timeline

Project Manager II, Research Manager

Seattle Children’s Hospital General Surgery Division
05.2022 - Current

Adjunct Assistant Professor

City University Of New York Baruch & Brooklyn
08.2021 - 12.2021

Non-Teaching Adjunct

City University Of New York Open Educational Resources Initiative (NSF Award #1827035)
12.2018 - 09.2019

Graduate Student Instructor

City University Of New York Brooklyn College
08.2013 - 05.2019

Genetics Intern

City University Of New York Sackler Institute For Comparative Genomics, American Museum Of Natural History
09.2012 - 05.2013

Graduate Research Assistant

State University Of New York Stony Brook
08.2010 - 07.2011

Genomics Specialist II

Fred Hutchison Cancer Research Center Genomics Shared Resources
10.2005 - 04.2009

Research Associate II

Xcyte Therapies, Inc. Research And Development Team
02.2004 - 08.2005

Genetics Lab Technician

Seattle Aquarium
03.2002 - 04.2009

AmeriCorps*VISTA

Panhandle Health District #1
09.1998 - 10.1999

Ph.D. - Ecology, Evolutionary Biology And Behavior

City University of New York Graduate Center

Master of Science - Marine & Atmospheric Science

State University of New York

Bachelor of Science - Cell & Molecular Biology

University of Washington
JIMIANE L. ASHE