Desired Job Title
Biotechnology Laboratory AssistantConsulting AssociateLocation Preference
RemoteOn-SiteHybridLocation: New York, NY
Open to relocation: Yes
Open To Work
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JUAN Idiarte
Work Preference
Overview
7
7
years of professional experience
Work History
Flow Cytometry Specialist
Columbia University
New York, United States of America
10.2024 - Current
- Handling, operation and configuration of flow cytometers and spectral and conventional cell classifiers (BD FACSDiscover S8, Sony ID7000, Sony MA900, FACSAria II, ImageStream MkII, Agilent Novocyte Penteon, BioRad ZE5), assisting users in their experiments and classifications in assays from 1 to 25+ fluorescence colors with human, animal, bacterial and viral samples.
- Handling, operation and support of 10X Genomics assays (5' and 3') for clinical trials in humans, tuning of assays and assistance in the creation of panels for flow cytometry experiments.
Biotechnology Laboratory Assistant
Universidad de la Republica
Montevideo, Uruguay
10.2023 - 10.2024
- Produced and purified bacterial pigments through primary cell culture techniques.
Executed recombinant protein production in bacterial systems.
Conducted qPCR to quantify gene expression levels.
Utilized flow cytometry for analysis of cell populations and characteristics.
Performed protein aggregation and disaggregation assays via Thioflavin T method.
Flow Cytometry Specialist
Columbia University
New York, United States of America
03.2023 - 10.2023
- Handling, operation and configuration of flow cytometers and cell classifiers (Sony MA900, FACSAria II, ImageStream MkII, Agilent Novocyte Penteon, BioRad ZE5), assistance to users in their experiments and classifications in assays from 1 to 25+ fluorescence colors with human, animal, bacterial and viral samples.
- Handling, operation and support of 10X Genomics assays (5' and 3') for clinical trials in humans, tuning of assays and assistance in the creation of panels for flow cytometry experiments.
Technician B
Columbia University
New York, United States of America
01.2022 - 03.2023
- Cell culture (human, mammalian and bacterial), virus production (AAV, lentivirus), primary cell cultures (human and mammalian), ELISA, rodent scanning with magnetic resonance imaging, flow cytometry, nucleic acid extraction, protein extraction, PCR, qPCR, western blotting, microscopy, tissue preparation, immunohistochemistry.
- Columbia University Irving Medical Center, New York, United States of America
Laboratory Assistant
Calister S.A (Lallemand Inc)
Canelones, Uruguay
10.2021 - 12.2021
- Conducted quality analysis of cell cultures using microscopy techniques.
Performed cell counting to assess bacterial growth in various assays.
Operated industrial plants to maintain optimal production environments.
Created and controlled bacterial initiators for research and production processes.
Assistant Researcher
Pasteur Institute
Montevideo, Uruguay
09.2020 - 08.2021
- Performed cell culture and executed flow cytometry for sample analysis.
Conducted exosome and microvesicle extraction to isolate cellular components.
Executed nucleic acid and protein extraction to prepare samples for further testing.
Applied PCR and qPCR techniques for quantitative analysis of genetic material.
Utilized western blotting for protein detection and validation of results.
Operated epifluorescence microscopy to visualize cellular structures and interactions.
Research Assistant
Universidad de la Republica
Montevideo, Uruguay
11.2019 - 12.2019
- Performed nucleic acid extraction and PCR for gene construct generation.
Executed cloning and targeted mutagenesis on Aspergillus nidulans.
Conducted plate growth analysis to assess genetic transformation outcomes.
Utilized Western blotting for protein expression analysis in transformed cultures.
Applied epifluorescence microscopy to visualize cellular structures.
Interpreted molecular simulation results to support experimental findings.
Final Degree Internship
Biochemistry Section
, Uruguay
04.2019 - 12.2019
- Nucleic acid extraction, PCR, gene construct generation, western blotting, cloning, targeted mutagenesis, Aspergillus nidulans culture and genetic transformation, plate growth analysis, epifluorescence microscopy, interpretation of molecular simulation results.
Education
MSc - Cell and Molecular Biology
Faculty of Sciences, UdelaR
Uruguay10-2024
Bachelor of Science - Biological Sciences
Faculty of Sciences, UdelaR
Uruguay06-2021
Skills
- Flow cytometry (Conventional and Spectral)
- Cell culture (iPSCs, Primary)
- Nucleic acid extraction
- Data analysis and experiment design
- Recombinant protein production
- qPCR techniques and RNA isolation
- Fluorescence microscopy
- Protein purification and expression
- Immunohistochemistry
- Chromatography techniques
- Molecular cloning
Personal Information
Date of Birth: 10/18/98
Place of Birth: Montevideo, Uruguay
Scientific Publications
2026
Sarmah, H., Iannello, G., Wantroba, R., Wu, C., Idiarte, J. F., Muñoz, A., Déry, O., de la Torre, E. R., Lebayle, E., Seminara, S., et al. (2026). Protocol for clonal isolation of gene-edited hiPSCs using droplet and microfluidic sorting. STAR Protocols, 7(2).
Tuddenham, J., Haage, V., Comandante-Lou, N., Bautista, A., Chiu, R., Fujita, M., Garcia, F. G., Bhattarai, P., Patel, R., Buonfiglioli, A., Idiarte, J.F., et al. (2026). A pipeline for drug repurposing identifies Topoisomerase-I inhibition as a potent regulator of human microglial phagocytosis and metabolism; extensions to psychiatric pharmacology. Biological Psychiatry, 99(10), S118.
2025
Arain, H. A., Mares, J. A., Sharma, A., Tedesco, T. G., Idiarte, J. F., Figueroa, H. Y., Guo, J., Duff, K. E., & Nuriel, T. (2025). APOE genotype modulates tau propagation in a novel humanized mouse model of early-stage Alzheimer’s disease. Alzheimer’s & Dementia, 21(Suppl 1), e106221.
2024
Haage, V., Tuddenham, Idiarte J. F., Comandante-Lou, N., Bautista, A., Monzel, A., Chiu, R., Fujita, M., Garcia, F. G., Bhattarai, P., Patel, R., et al. (2024). A pharmacological toolkit for human microglia identifies Topoisomerase I inhibitors as immunomodulators for Alzheimer’s disease. bioRxiv (preprint).
Arain, H. A., Idiarte, J. F., Lama, T. D., Figueroa, H. Y., Guo, J., Duff, K. E., & Nuriel, T. (2024). APOE4 alters tau propagation in regions associated with early Alzheimer’s disease in a humanized mouse model of tau spread. Alzheimer’s & Dementia, 20, e089837.
2022
Sanguinetti, M., Silva Santos, L. H., Dourron, J., Alamón Lima, C. I., Idiarte, J. F., Amillis, S., Pantano, S., & Ramón, A. (2022). Substrate recognition properties from an intermediate structural state of the UreA transporter. International Journal of Molecular Sciences, 23(24), 16039.
Sanguinetti, M., Santos, L. H., Dourron, J., Alamón, C., Idiarte, J. F., Amillis, S., Pantano, S., & Ramón, A. (2022). An AlphaFold2 intermediate conformational state provides insights on urea transporter function. SSRN (preprint).
2021
Sanguinetti Miralles, M., Ramón Pacheco, A., Amillis, S., Pantano Gutierrez, S. F., Idiarte, J. F., Dourron Fernández, J., & Alamón Lima, C. I. (2021). Informe final del proyecto: Profundización en la identificación de determinantes estructurales y funcionales de UreA. Agencia Nacional de Investigación e Innovación.
2019
Poster presentation at the second National Congress of Biosciences, Montevideo, "Deepening the identification of structural and functional determinants of UreA (#131)"
Awards
INJU Award Winner, Montevideo, 10/01/20, Category "Science, innovation and environment" for performance in the area of science.
Languages
English
Native/ Bilingual
Spanish
Native/ Bilingual
Italian
Native/ Bilingual
Portuguese
Native/ Bilingual
Timeline
Flow Cytometry Specialist
Columbia University
10.2024 - Current
Biotechnology Laboratory Assistant
Universidad de la Republica
10.2023 - 10.2024
Flow Cytometry Specialist
Columbia University
03.2023 - 10.2023
Technician B
Columbia University
01.2022 - 03.2023
Laboratory Assistant
Calister S.A (Lallemand Inc)
10.2021 - 12.2021
Assistant Researcher
Pasteur Institute
09.2020 - 08.2021
Research Assistant
Universidad de la Republica
11.2019 - 12.2019
Final Degree Internship
Biochemistry Section
04.2019 - 12.2019
MSc - Cell and Molecular Biology
Faculty of Sciences, UdelaR
Bachelor of Science - Biological Sciences
Faculty of Sciences, UdelaR