Kayla Hatashita
Bonita,CA
Summary
Highly-motivated, recently graduated college student with a Bachelor of Science in Molecular, Cell, and Development Biology and extensive background in the food industry, eager to transition into a laboratory position. Using skills learned through education and restaurants, I can learn quickly and adapt when new material is presented. Proven ability through food service and labs to follow strict protocols, maintain composure in fast-paced environments, and complete tasks in a timely manner independently. Now seeking to utilize transferrable skills and passion for molecular biology to make meaningful contributions in a laboratory setting.
Overview
2
2
years of professional experience
Work History
Server
The Butcher Shop Steakhosue
San Diego
06.2022 - Current
- Provided excellent customer service to ensure satisfaction.
- Assisted in training new servers.
- Maintained knowledge of current menu items, garnishes, ingredients and preparation methods.
- Took orders from customers accurately and in a timely manner.
- Checked food before serving it to customers.
- Responded efficiently to guest inquiries and complaints in a professional manner.
- Ensured that each guest was served courteously, quickly, and efficiently.
Identification of Unknown Sample
University Of California, Santa Cruz (Senior Exit)
Santa Cruz, CA
07.2023 - 09.2023
- Sterile collection of sample using saline solution.
- Knowledge of Luria Broth as a non-selective and non-differential medium.
- Proper plate-streaking technique to yield discrete colonies in DNA purification.
- Inoculation of liquid cultures.
- DNA extraction by cell lysis.
- Gram staining and analysis of results.
- PCR and gel electrophoresis
- Kirby Bauer Assay for antibiotic susceptibility.
- Performance of biochemical assay.
- Identified sample as Paenibacillus xylanivorans.
Formation of Recombinant Plasmid PET-41a(+)/egfp
University Of California, Santa Cruz
Santa Cruz, CA
04.2023 - 06.2023
- Gel electrophoresis of ligation reactions.
- Transformations using E.coli on Kanamycin and IPTG plates.
- Prediction of gene expression prior to streaking.
- DNA purification and use of nanodrop spectrophotometer to check success.
- Restriction digest using NotI and NcoI
- PCR to amplify recombinant plasmid
- Western Blotting to prove presence and isolation of GST-egfp.
- Sonication and affinity chromatography for protein expression.
- Bradford Assay for protein concentration in sample.
- Successful formation of recombinant plasmid.
Education
Bachelor of Science - Molecular, Cell, And Developmental Biology
University of California, Santa Cruz
Santa Cruz, CA09-2023
Skills
- Specimen Handling
- PCR
- Pipette accuracy
- Attention to Detail
- Time Efficiency
- Communication
- Team Collaboration
- Sample Collection Techniques
- Knowledgeable In Molecular Biology
- Sterilization Techniques
- Sample Handling
- DNA Testing And Analysis
Affiliations
- Musical Theatre (11 years)
- Gymnastics (8 years)
- Portuguese-American Dancing (14 years)
Accomplishments
- University Grant
- UPSES Scholarship
- Portuguese Historical Center Scholarship
Languages
English
Full Professional
Spanish
Elementary
Portuguese
Native/ Bilingual
References
References available upon request.
Timeline
Identification of Unknown Sample
University Of California, Santa Cruz (Senior Exit)
07.2023 - 09.2023
Formation of Recombinant Plasmid PET-41a(+)/egfp
University Of California, Santa Cruz
04.2023 - 06.2023
Server
The Butcher Shop Steakhosue
06.2022 - Current
Bachelor of Science - Molecular, Cell, And Developmental Biology
University of California, Santa Cruz
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