Mary Koroma

Undergraduate Laboratory Coursework

Pennsylvania State University

• Performed PCR (polymerase chain reaction) for DNA amplification, including reaction setup, thermal cycling, and troubleshooting
• Conducted agarose gel electrophoresis to separate and analyze DNA fragments, including gel preparation and interpretation of band patterns
• Carried out protein purification techniques (e.g., column-based methods) to isolate target proteins and evaluate sample purity
• Applied aseptic technique for microbial culturing, isolation, and contamination prevention
• Executed molecular biology workflows, including DNA/RNA handling, precise micropipetting, and experimental setup
• Performed biochemical and enzyme assays, analyzing reaction rates and interpreting experimental results
• Prepared chemical solutions and performed quantitative analyses, including dilutions and molarity calculations
• Maintained detailed and organized laboratory notebooks documenting procedures, data, and conclusions
• Followed strict laboratory safety protocols, including proper PPE use, chemical handling, and waste disposal