Pallavi Gadre
Fremont,CA
Summary
- Lab Assistant / Technician/ Research Associate Overview Accomplished Bio manufacturing Certification and studying toward the bachelor’s program to work in research. Have done courses toward a pre-medical science degree. I have experience with Fermentation, Media, Greenhouse, Genomics, Bioinformatics, and BioRepository teams. Prepared media for large batch fermentation used for flask and tank inoculation, maintained sterile level in BioSafety, and created sterile sampling and inoculation. Maintained sterile level in BioSafety including Aseptic sampling and aliquoting hazardous biological materials following skills adhered to following SOPs and GMP. Accuracy in writing and creating documents with appropriate documentation following protocols. Detailed experience in cell culture for research and development with an interpretation of data. Using Bioinformatics and data analysis for concluding the research project. Hands-on experience in human physiological laboratory skills with human tissue and blood sample handling. Have also done instrumental procedures, calibration, and maintenance of a variety of lab equipment. Operated a large volume of BioRepository inventory. Worked independently and collaboratively in a team, taking the initiative to lead the team. My Strength:
- Goal-oriented professional, with exceptional laboratory knowledge and skills in biotechnology, with a strong interest in fermentation, microbiology, and experimental research and manufacturing.
- Good interpersonal skills and the ability to consistently project a positive and professional attitude in a team.
- Always been a self-starter with a strong work ethic, organizing skills, and adaptability.
- Accurate knowledge of MS office for data input, statistics, and data analysis.
- Specialized in troubleshooting complex problems through detail-driven practice and identifying correct solutions to the problem.
- Fast learner and collaborative professional, who can contribute to an organization's growth and success by working independently and leading a team.
Overview
20
20
years of professional experience
Work History
Associate
Ohlone College
06.2023 - 08.2024
- Studying the courses towards an, degree in Biotechnology
- Had course in: Genomics: Learned to construct, search, and analyze simple genomic and cDNA libraries
- Developed library construction techniques, replica plating, and the use of non-radioactive oligonucleotide probes for searching libraries to find DNA sequences of interest
- Additionally, performed DNA modification techniques and how to construct a simple restriction map from electrophoresis gel data
- Bioinformatics: Computational techniques to address current biological problems such as the SARS-CoV-2, and COVID-19
- Worked on DNA, RNA, and protein sequence analysis
- Did prediction of protein structure, analyze data from biological databases, apply genome annotation methods, build phylogenetic trees from sequence data, and predict gene function based on molecular data
- HPLC: It is used to separate and analyze compounds through the mass-transfer of analytes between stationary and mobile phases 1-3.
QC Inventory Technician
Thermo Fisher Scientific
Fremont, CA
09.2022 - 05.2023
- Maintaining the pharmaceutical inventory used for testing in the QC lab
- Maintains material transactions involving quality control and research-related components
- Create reports to analyze inventory and set up inventory spreadsheet
- Conducted quarterly inventory cycle counts to maintain inventory accuracy
- Daily logging of the products in the inventory and the QC log
- Set up and print inventory lists for audit
- Processing Retain requests for the QC and non-QC requirements
- Troubleshooting for retaining requests if the components are not available.
BioRepository Technician
Roche Molecular Solutions
Pleasanton, CA
08.2019 - 06.2020
- Performs BioRepository Management duties for BioSpecimen Management, with meticulous attention to details
- Works independently to receive shipments of human biological samples, verify sample integrity and data accuracy
- Coordinate and harmonize the tasks of quality assurance in the laboratory
- Control of the preparation and update of the documentation
- Choose partners for delivering laboratory equipment and constantly supervise the purchase
- Process (centrifuge, aliquot, and pool) samples, consolidate inventory, retrieve and distribute samples, ship specimen collection supplies to partners, and monitor laboratory supply inventory
- Duties include handling hazardous biological materials (BSL-2 and BSL-3)
- Contributed to maintaining Key Performance Indicators (KPI), suggesting refinements in a dynamic environment
- Maintained a high level of organization to independently manage all aspects of complex service requests at the program level and think creatively to resolve challenges as encountered
- Provided input to improve existing laboratory procedures
- Assisted in the development and implementation of standard operating procedures
- Participated in required training related to the position; take personal responsibility for being current in all training activities.
Lab Assistant
Mendel Biological Solutions
Hayward, CA
09.2018 - 04.2019
- Assisted the Fermentation, Media, and Greenhouse teams to plan operations and implementation of experiments by maintaining accurate practices and a safe work environment
- Following are the key tasks performed:
- Maintain sterile level in BioSafety including Aseptic sampling and aliquoting skills
- Manage inventory, and update stock by ordering & delivering laboratory supplies and managing shipments
- Exercise the skills to maintain a detailed protocol for documentation of the experimental data accurately following Standard Operating procedures standards
- Assisted and independently created SOPs
- Work Independently towards the lab task, interpreting data and getting results
- Independently perform the troubleshooting and work toward constant improvement and innovation of procedures to enhance laboratory workflow
- Develop assay kits and designed projects with planning to execute it
- Operate pH meter, Brix, electronic balance/ digital scale, HPLC, and YSI equipment with regular maintenance and calibration
- Prepare media for large batch fermentation used for flask production and tank inoculation
- Maintain cell bank vials including destroying them after inoculation
- Did effective sampling of cultures and delivered the samples to testing and further execution
- Skillfully conducted accurate microbial cell culturing with streaking for singles and liquid cultures
- Studied quantification of gene expression by qPCR and the role of RNA
- Implementing chemical and molecular biology test methods that have been developed by labs
- Protein purification is performed to processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues, or whole organisms
- Pour plates with various dimensions using a media dispenser and manually
- Perform operations in a clean room with standard 100 and 100, Clean-In-Place (CIP), and Steam-In-Place (SIP)
- Performed tasks in Greenhouse which include Potting and Planting, Herbicide study, and Biomass measurement.
Courtesy Clerk
Railey’s Supermarket
Fremont, CA
09.2017 - 04.2018
- Provided customer service and problem-solving solutions
- Periodically check on the product availability status
- Performed inventory of the various items and developed a list of product orders.
Ohlone College
Newark, CA, CA.
01.2014 - 06.2017
- Hands-on experience in the laboratory while in the Biomanufacturing program at Ohlone College, Newark
- Performed accurate pipetting and Micropipetting skills during solution making to ensure appropriate reaction to experiment
- Prepared gels and ran agarose gel electrophoresis and skilled calculation of percent & Standard Deviation
- Maintained a clean room environment, clean room gowning, and apply MSDS and practiced Implementation of GMP, GXP, SISPQ, SOP, and Quality guidelines
- Studied quantification of gene expression by qPCR and role of RNA and operated DNA Sequencer and PCR
- Implementing chemical and molecular biology test methods that have been developed by labs
- Performed procedures and maintained lab equipment like Autoclave, Hot bath, Compound light microscope, HPLC, Incubator, micropipette, Centrifuge, petri dish, electronic balance/ digital scale, Bunsen burner, Laminar air flow chamber, fume hood, microcentrifuge tubes, and electrophoresis chamber.
Volunteer
Fremont Unified School District, FUSD
Fremont, CA
04.2017 - 06.2017
- Volunteered in FUSD’s Oliveira Elementary school by performing tasks to organize the events for the school events and assisting in the library and school closets inventory.
Associate
Amazon Sorting Center
Newark, CA
11.2016 - 03.2017
- Worked as an Amazon Associate at a sorting center
- Performed sorting of the incoming shipment and getting it ready for delivery.
Liaison
Kaiser Permanente
Fremont, CA
06.2006 - 03.2009
- Worked as Liaison for the surgical department
- Assisted with the communication between the surgical team and the patient’s relatives.
Volunteer
Music for Minors
Fremont, CA
02.2005 - 01.2006
- Volunteered to run the nonprofit music program in public schools.
Education
Associate Degree - Biotechnology
Ohlone College
01.2024
Bio Manufacturing Certification -
Ohlone college
01.2016
Associate degree - pre
Medical College
01.2001
bachelor’s degree - Biochemistry
High School Diploma - Molecular Biology
Shailendra Education Society
Timeline
Associate
Ohlone College
06.2023 - 08.2024
QC Inventory Technician
Thermo Fisher Scientific
09.2022 - 05.2023
BioRepository Technician
Roche Molecular Solutions
08.2019 - 06.2020
Lab Assistant
Mendel Biological Solutions
09.2018 - 04.2019
Courtesy Clerk
Railey’s Supermarket
09.2017 - 04.2018
Volunteer
Fremont Unified School District, FUSD
04.2017 - 06.2017
Associate
Amazon Sorting Center
11.2016 - 03.2017
Ohlone College
01.2014 - 06.2017
Liaison
Kaiser Permanente
06.2006 - 03.2009
Volunteer
Music for Minors
02.2005 - 01.2006
Associate Degree - Biotechnology
Ohlone College
Bio Manufacturing Certification -
Ohlone college
Associate degree - pre
Medical College
bachelor’s degree - Biochemistry
High School Diploma - Molecular Biology
Shailendra Education Society
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