Rouwaida O. Nitiema

Advisor: Dr. Sabrina Scroggins

• We are focused on expanding our knowledge on the characterization of inflammatory immune maternal microchimeric cells in preeclampsia-exposed offspring, such as TH17, using flow cytometry analysis
• We aim to determine whether TH17 mMCs and IL-17 signaling during preeclampsia-affected pregnancy are required for long-term alterations in offspring TH dysfunction.
• We also aim to determine whether TH17 mMCs that persist postnatally contribute to sustained IL-17 production and offspring TH immune and behavioral dysregulation.

• Assisted in implementing experimental protocols (Migration and Invasion Assays of HTR8 and JEG3 Trophoblast cell lines) for data collection.
• Analyzed research data using statistical software (GraphPad Prism) to ensure accuracy and consistency.
• Trained fellow lab members in cell culture, migration, and invasion assay techniques.
• Planned, modified, and executed research techniques and procedures: HTR8 and JEG3 migration and Invasion experiments.
• Conducted extensive background research on Maternal-fetal microchimerism, Preeclampsia, and Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), establishing a strong foundation for project success.
• Recorded data and maintained source documentation following good documentation practices.
• Worked as member of integrated project team in highly collaborative work environment.
• Worked as a member of an integrated project team in a highly collaborative work environment.ment.t.riments under the supervision of senior lab members.

• Managed and prepared documentation for investigational drugs for pharmacists on various study protocol
• Accurately collected, maintained and organized data and files, according to the study protocols
• Data entry and data management
• Collect, process, and ship biological samples
• Managed participant recruitment and maintained databases for research tracking purposes.
• Participated actively in regular meetings with fellow researchers to discuss project updates, challenges faced, and lessons learned during ongoing activities.
• Organized research materials, maintaining a well-ordered workspace conducive to productivity.
• Maintained open lines of communication with project leads and team members for effective collaboration on common goals.
• Participated in lab meetings and project presentations.
• Troubleshot and resolved equipment and process issues.

• Course Tutor for Molecules to Medicine and Hematology—Oncology
• Prepared lesson plans and conducted weekly course tutoring sessions.
• Implemented innovative teaching techniques to accommodate diverse learning styles and needs of students.

• Genetic analysis on how mutations in two genes relieve defects in a novel fungal microtubule-stabilizing protein.

• Generated mutations in the novel fungal protein, WDA.

• Analyzed suppressor mutations through linkage analysis genetics.

• Determined the dominance and recessive nature of WDA suppressor mutations.

• Studied the epistatic relationship between mutations in two of the WDA suppressor genes.

• Determined the dominant and Recessive nature of WDA suppressor mutations and linkage analysis.

• Worked closely with faculty (Professor C. Biggins, Professor L. Reinhart)
• Ran supplemental lab Review sessions.
• Helped students through group and one-on-one tutoring.
• Prepared the specimens and equipment needed for each lab lecture.
• Implemented differentiated instruction strategies to meet diverse learning needs.
• Identified struggling students early on and provided additional support through tutoring sessions and modified lesson plans.

• Researched the physiological roles of pandemic Vibrio cholerae at the Aux3 Locus with Dr. Pukatzki

• Conducted genetic analyses: microarray, qPCR, genomic sequencing, and Sanger sequencing.

• Performed motility/swarm assays on mutant strains of pandemic Vibrio cholerae.

• Inoculated, measured the optical density, and plated Vibrio cholerae cells.

• Performed DNA and plasmid extractions.

• Ran gel electrophoresis and western blots.

• Generated clones of mutant Vibrio cholerae strains through Gibson Cloning.

• Performed Transmission Electron Microscopy (TEM) for analysis of cultured Vibrio cholerae strains.

• Learned how to utilize aseptic techniques in a laboratory setting.

• Conducted individual research on the morphology of Collagen-Cellulose Biofilms with Dr. David Salas
• Regenerated biofilms using varying ratios of Collagen-Cellulose, and coagulation mediums.
• Characterized Biofilms using FT-IR, SEM, TGA, and DSC analyses.
• Managed time efficiently in order to complete all tasks within deadlines.
• Organized and detail-oriented with a strong work ethic.
• Paid attention to detail while completing assignments.
• Used critical thinking to break down problems, evaluate solutions, and make decisions.
• Strengthened communication skills through regular interactions with others.
• Learned and adapted quickly to new technology and software applications.