With a Masters in Industrial Engineering and over 30 years of experience in a Neuroscience research laboratory, where my responsibilities include but no limited to Cell culture, working with different types of primary cells and cell lines,;
Immunocytochemistry; imaging; lab management . Working effectively and collaboratively with different labs.
Development and Characterization of Schwann Cell Derived Exosome production.
Determine the efficacy of exosome treatments (SC and MSC) to enhance axonal growth in embryonic rat DRG-derived purified neurons and whole DRGs
carry out in vitro assays with purified neurons and whole DRGs of Sprague-Dawley embryonic rat (E15), determine exosome batch-to-batch or donor- to-donor effects on axonal growth, dose-response effects and differences in SC- and MSC-derived exosome treatments.
Interact with establish group of research scientist in studies of the regulation of Rat and Human Schwann cells function, including mechanisms regulation interactions with extracellular matrix proliferation and differentiation. Prepare the supply of rat, primate and/or human Schwann cells for transplantation
Keeping bank of different types of primary cells, maintenance for Cryoplus machines.
Developed culture conditions for studying the differentiation and proliferation of stem cells from rat and human brain and spinal cord in vitro. Identification of their degree of differentiation and phenotype thought the use of immunochemistry
Identification and analyzed sources of chemical pollution for pharmaceutical industry., presenting recommendations for technological improvements.
Nishimura K, Sanchez-Molano J, Kerr N, Pressman Y, Silvera R, Khan A, Gajavelli S, Bramlett HM, Dietrich WD.
Beneficial Effects of Human Schwann Cell-Derived Exosomes in Mitigating Secondary Damage After Penetrating Ballistic-Like Brain Injury.
J Neurotrauma. 2024 Apr 15. doi: 10.1089/neu.2023.0650..
Stratton S, Wang S, Hashemi S, Pressman Y, Nanchanatt J, Oudega M, Arinzeh TL.
A scaffold containing zinc oxide for Schwann cell-mediated axon growth.
J Neural Eng. 2023 Nov 17;20(6). doi: 10.1088/1741-2552/ad0a00.
Paudyal S, Vallejo FA, Cilingir EK, Zhou Y, Mintz KJ, Pressman Y, Gu J, Vanni S, Graham RM, Leblanc RM
DFMO Carbon Dots for Treatment of Neuroblastoma and Bioimaging.
ACS Appl Bio Mater. 2022 Jul 18;5(7):3300-3309. doi: 10.1021/acsabm.2c00309. Epub 2022 Jun
Khan A, Diaz A, Brooks AE, Burks SS, Athauda G, Wood P, Lee YS, Silvera R, Donaldson M, Pressman Y, Anderson KD, Bunge MB, Pearse DD, Dietrich WD, Guest JD, Levi AD.
Scalable culture techniques to generate large numbers of purified human Schwann cells for clinical trials in human spinal cord and peripheral nerve injuries.
J Neurosurg Spine. 2021 Sep 3;36(1):135-144. doi: 10.3171/2020.11.SPINE201433. Print 2022 Jan 1.
Puhl DL, Funnell JL, D'Amato AR, Bao J, Zagorevski DV, Pressman Y, Morone D, Haggerty AE, Oudega M, Gilbert RJ.
Aligned Fingolimod-Releasing Electrospun Fibers Increase Dorsal Root Ganglia Neurite Extension and Decrease Schwann Cell Expression of Promyelinating Factors.
Front Bioeng Biotechnol. 2020 Aug 14;8:937. doi: 10.3389/fbioe.2020.00937..
Kanno H, Pressman Y, Moody A, Berg R, Muir EM, Rogers JH, Ozawa H, Itoi E, Pearse DD, Bunge MB
Combination of engineered Schwann cell grafts to secrete neurotrophin and chondroitinase promotes axonal regeneration and locomotion after spinal cord injury.
.J Neurosci. 2014 Jan 29;34(5):1838-55. doi: 10.1523/JNEUROSCI.2661-13.2014.
Zhang L, Ma Z, Smith GM, Wen X, Pressman Y, Wood PM, Xu XM.
GDNF-enhanced axonal regeneration and myelination following spinal cord injury is mediated by primary effects on neurons.
Glia. 2009 Aug 15;57(11):1178-91. doi: 10.1002/glia.20840.
Pearse DD, Sanchez AR, Pereira FC, Andrade CM, Puzis R, Pressman Y, Golden K, Kitay BM, Blits B, Wood PM, Bunge MB.
Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery.
Glia. 2007 Jul;55(9):976-1000. doi: 10.1002/glia.20490.
Grimpe B, Pressman Y, Lupa MD, Horn KP, Bunge MB, Silver J.
The role of proteoglycans in Schwann cell/astrocyte interactions and in regeneration failure at PNS/CNS interfaces.
Mol Cell Neurosci. 2005 Jan;28(1):18-29.
Plant GW, Currier PF, Cuervo EP, Bates ML, Pressman Y, Bunge MB, Wood PM.
Purified adult ensheathing glia fail to myelinate axons under culture conditions that enable Schwann cells to form myelin.