Jana' M. Ennis

• Utilize microtome to prepare very thin sections of human tissue, bone marrow and biopsies.
• Team Lead in performing all basic functions of Histotechnician: processing, embedding, microtomy, routine Hematoxylin and Eosin (H&E) staining; manual staining, non-silver staining techniques (such as Giemsa, Brown-Hopps (B&H), Iron and Copper reactions) special and immunohistochemistry staining.
• Archive, catalog, scan and file hundreds of slides while identifying quality of stains on slides using electron microscope increasing productivity.
• Use spreadsheets and database for cataloging and filing as well as organize slides for long-term storage and other duties as required.
• Team Lead in identification and discerning nature of stained slides, relabeling, scanning, archiving and cataloging. Utilizing photo-editing software and preparing digital images, improving turn around times 25%.
• Examine slides under microscope to detect deviations from normal and report abnormalities.
• Organize over 300 slides into appropriate staging and storage locations while maintaining movement and tracking logs or documents.
• Archive over 300 slides daily for long-term storage by identifier in JPC's Repository.
• Establish and maintain user friendly electronic systems (Excel or another electronic database) for patient slides and archival research.
• Provided training for new staff members, sharing expertise in histology procedures and best practices.
• Collaborated with pathologists to ensure accurate diagnoses, assisting in examination of tissue samples.

• Proficiently perform laboratory techniques for processing and testing specimens, following Standard Operating Procedures (SOPs). Team Lead in performing all laboratory procedures.
• Process Tissues H&E Stains Specials Stains
• Immunohistochemistry Embedding Microtomy/Cutting
• Accession Specimens Coverslip Label Slides (Shur Mark)
• Grossing
• Independently perform as Lead Histotechnician staining patient tissues/biopsies with routine Hematoxylin & Eosin and Immunohistochemical (IHC) stains while understanding chemical reactions of certain tissues and cells to establish correct results for diagnosis, didactic and research purposes.
• Conduct Quality Control procedures on Immunohistochemistry (IHC) reagents, equipment (DAKO, Ventana, Leica); and maintain records for quality control reports and College of American Pathologist/Clinical Laboratory Improvement Amendments (CAP/CLIA) requirements.
• Utilize microtome to prepare thin sections of human tissue, bone marrow and biopsies.
• Obtain and validate control material used in (IHC) procedures.
• Accurately prepare slides from tissue sections for microscopic observation and diagnosis.
• Team Lead in performing use and care of automated equipment and solution inventory; utilize Biological Safety Cabinet (BSC), Liquid Nitrogen, Centrifuge, Pipettes.
• Setup tissue processors, water baths, stain lines and cover slipper.
• Participated in quality control activities: staining, reviewing slides and ensure quality.

Proficiently perform histology preparation of frozen tissue section(s) required during surgery. Duties included: mapping, dividing and dying cut edges of tissue, embedding tissue in mounting medium for horizontal orientation.

• Processing frozen tissue sections using cryostats, mounting the sections on labeled slides, staining sections with routine Hematoxylin and Eosin (H&E) staining; also manual staining, nonsilver staining techniques (such as Giemsa, Brown-Hopps (B&H), Iron and Copper reactions) or Special Stains and cover slipped microscopic slides.
• Evaluate stains and slide quality microscopically to determine clinical diagnosis by anatomic pathologist.
• Review quality control documents to identify problems and establish corrective actions. Ensuring quality results associated with diagnostic testing.
• Process frozen tissue sections, cutting multiple levels ensuring complete removal of skin cancer while preserving normal tissue.
• Performs highly specialized techniques with delicate and complex tissues.
• Performs exceptionally well, independently and as Team Lead.
• Lead in performance of daily operational tasks; preventive maintenance and calibration of equipment and sterilization of work areas.
• Accession specimens into database.
• Maintain inventory and orders laboratory supplies.
• Biological Safety Cabinet (BSC), centrifuge, pipettes and liquid nitrogen.
• Handle, fix and prepare specimens.
• Prepare frozen sections of delicate tissue from all parts of body for Basal (BCC) and Squamous Cell Carcinomas (SCC) for cancer diagnosis.
• Apply clinical pathology rules and regulations, and review slides for analysis.
• Team Lead in operating and troubleshooting laboratory equipment, instruments and repair cryostats to prepare frozen sections for rapid diagnosis of cancer.
• Document over 100 test results daily into electronic laboratory information system (LIS).
• Assist in operating room and lab, communicating with surgeons and patients.
• Assist with patient prep, surgical supplies, equipment and suture removal.
• Assists with Research & Development activities: prepare tissues and slides for research Biobank (cryopreserve samples) and maintains quality control records.

• Applied standard prescription formulas to calculate dosages.
• As Lead Technician prepared medications, suspensions, ointments and creams utilizing Baker Cells dispenser and trained others.
• Ordered and maintained inventory, supplies and purchasing procedures.
• Update and maintain profiles in database.
• Performed insurance billing, prescription interpretation and utilized Rx Connect pharmacy's computer program to access prescription information.
• Thwarted potentially harmful drug interactions, insurance issues and other matters to ensure patient safety.

• Assume leadership roles and trained others in laboratory processes. Histology tasks performed were embedding, cutting with microtome (Leica), staining paraffin embedded tissues with special Histochemical Stains, Hematoxylin and Eosin (H&E) and Immunohistochemistry stains (IHC).
• Prepare surgical and frozen specimens for microscopic observation. Maintained and followed standard operating procedures (SOPs) and good laboratory practices (GLPs) for quality control.
• Performs highly specialized techniques such as direct Immunofluorescence, In-Situ-Hybridization. Some of special stains performed included: Periodic Acid-Schiff Stain (PAS), Chloroacetate Esterase Stain (Leder), Masson's Trichrome Stain (TRI), Periodic Schiff-Methenamine Silver (PASM), Congo Red, Grocott's Methenamine Silver Stain (GMS).
• Maintained and implemented variety of routine and specialized laboratory equipment: Automatic Stainers (Ventana and Artisan), Tissue Processors (Sakura Tissue-Tek), Embedding Station (Sakura Tissue-Tek TEC 5), Cover slipper (Leica ASP 300) and Laboratory Information System (LIS), CoPath.
• Ordered and inventoried laboratory supplies and equipment to ensure compliance and regulation factors were maintained.
• Successfully perform standard analysis of pathogen data and gather information/research to recognize variants in positive and negative tissue specimen; Basal (BCC) and Squamous Cell Carcinomas (SCC) for cancer diagnosis.
• Coordinate and distribute biopsies and tissue specimen daily.
• Prepared slides of tissues, fixed and prepared specimens received utilizing aseptic techniques to increase lab's productivity.
• Prepared reagents and solutions to enable machines responsible for processing specimens embedding and staining.
• Generated reports to pathologists for emergency surgical procedures.
• Utilize Co-Path Laboratory Information Management Systems (LIMS) to enter data into Quality Control Management System (QCMS).
• Adapt technical procedures for several different variables in process of fixing and staining tissue.

• Certified Pharmacy Technician with proficiency performing pharmacy related activities, leading and training others in pharmaceutical inventory and data entry; good customer service skills and knowledge of specialized computer systems and programs.
• Prepared medication, suspensions, ointments and creams utilizing aseptic techniques to prevent microbial reaction due to physical and chemical factors in environment; and operated automated Baker Cell dispenser.
• Apply standardized formulas and procedures in weighing, measuring and mixing quantities of pharmaceuticals.
• Processed insurance performed billing and prescription interpretation; and utilized Rx Connect pharmacy information systems to track prescriptions and drug availability.
• Prepare and disseminate STAT orders.
• Provide on-the-job training to new employees.
• Conduct inventory of pharmaceutical stock, order medication and other supplies to ensure compliance and efficient operation.
• Trained new pharmacy technicians on proper procedures and best practices, ensuring consistent quality of service.
• Answered incoming phone calls and addressed questions from customers and healthcare providers.
• Consulted with insurance company representatives to complete claims processing, resolve concerns, and reconcile payments.
• Resolved non-routine issues like third-party billing, computer system, and customer service issues.
• Reviewed and verified customer information and insurance provider information.

• Leadership role in proficiently performing laboratory techniques for processing and testing clinical specimens that require specialized handling and reporting, while following Standard Operating Procedures (SOPs).
• Perform histology procedures with human tissue sections by processing, fixing and preparing Gyn and Non-Gyn specimens; embedding, cutting and staining paraffin embedded/frozen tissue with Hematoxylin and Eosin (H&E) and special stains for patient diagnosis and treatment.
• Some of special stains performed included: Periodic Acid-Schiff Stain (PAS), Chloroacetate Esterase Stain (Leder), Masson's Trichrome Stain (TRI), Periodic Schiff-Methenamine Silver, (PASM), Congo Red, Grocott's Methenamine Silver Stain (GMS).
• Accession data and forward reports to pathologists.
• Coordinate and distribute biopsies, tissue and specimen daily.
• Accurately prepared slides from tissue sections and other body fluids for microscopic observation and diagnosis.
• Team Lead and train others in performance of techniques, care of automated equipment and solutions; inventory and purchasing procedures.
• Setup tissue processors, water baths, strainers, cover slips and other instruments.
• Performed routine laboratory procedures such as analysis of blood, urine and other body fluids.
• Participated in quality control activities: staining, reviewing slides and ensure quality.
• Maintains, orders and stocks laboratory reagents, antibodies, equipment and supplies.
• Achieved faster results for clinicians by prioritizing urgent specimens as needed for rapid processing.

• Specialized experience as Microbiology Technician leading and training others in utilizing application and theory of microbiology techniques; in accordance with (FDA) and (USDA) compliance and regulations for food and safety.
• Performed microscopic observations of microorganisms to characterize different strains of harmful bacteria in dairy and food products.
• Evaluated dairy samples for harmful levels of Escherichia coli O: 157, Salmonella, Coliform and Gram positive bacteria contamination in samples; utilizing direct microscopic analysis (scanning electron microscope) and standard plate count.
• Coordinate rotation for testing milk samples prior to accepting products.
• Perform chemical analysis- (mass spectrometry) (MS) to identify plant protein added to milk products, positively distinguished adulterated milk samples from authentic milk.
• Collected and prepared samples for analysis in accordance with standard operating procedures and quality control requirements.
• Perform biological techniques: Enzyme-Linked Immunosorbent Assays (ELISA) to test for animal Trypanosoma: cell culture and microscopic observations to evaluate T. vivax along with different strains of bacteria in animal dairy and food products.
• Administered and enforced quality assurance procedures in laboratory. Utilizing aseptic techniques, standard operating procedures (SOP's) and good laboratory practices (GLP's).
• Perform variety of chemical, physical and microbiological analysis on aqueous and non-aqueous samples.
• Monitored farmer's dairy samples for compliance with timelines and temperature; observation of sample conditions not limited to color, odor, total hardness, conductivity, turbidity, pH, dissolved oxygen, solids, alkalinity, fluoride, chlorine and results of rapid test.
• Recorded, analyzed, and interpreted data.

• Utilized health and sanitation practices while performing quality control analysis: Western Blot Analysis and HIV-1 Urine Enzyme Immunoassay test routinely to validate results of samples, mass spectrometry (MS); and conducted Environmental test.
• Analyzed data and made conclusions by identifying and detecting problems with quality of Western Blot test (WB) and HIV-1 Urine Enzyme Immuno-Assay (EIA).
• Water quality assessment and monitoring to maintain quality control practices in laboratory, and standard operating procedures for testing.
• Observed positive results, checked appearance of weak and faded bands on Western Blot test strips and made modifications.
• Detected unsatisfactory trends and weaknesses in quality of Western Blot test strips.
• Reviewed information for outgoing documents containing laboratory and test results, working in accordance with established policies and good laboratory practices.
• Recorded and entered experimental data; and created graphs using Microsoft Word Software.

• Participated in Marine Fish Pathology research studies to determine fish diseases and cancer in Striped Bass and assist with obtaining grants for special research projects at University of Maryland Eastern Shore.
• Responding to requests for information regarding projects for grants and future aspirations.
• Assist in preparing science related events.
• Collecting information pertaining to public health and fish pathology.
• Microscopically analyzed tissue samples of Striped Bass.
• Lead colleagues in performing immunology test (Gel Electrophoresis and (PCR) DNA sequence analysis and studies.
• In addition to my research, maintained databases to document my results.
• Review board found Marine Fish Pathology research studies to be beneficial and eligible for grant funding.

As Physical Science Aide my duties consisted of supporting nutrition health and nutrition assistance programs. Moreover, food purchasing, acquiring recipes, gathering nutritional data, surveying and researching to assist with product analysis for the National Food and Nutrient Analysis Program (NFNAP).

• Conducting tests on food additives and preservatives to ensure compliance on such factors as color, texture and nutrients.
• Performed standardized qualitative and quantitative tests to determine physical properties of food or beverage products, to ensure compliance with quality standards.
• Sampled food products for taste or smell for prescribed flavor.
• Computed ingredient factors such as salt content, moisture, residue, or solubility.
• Analyzed records and compiled test results in database.
• Collaborated with Scientists and Nutritionists.