JOSEPH BILLANES
Escondido,California
Overview
8
8
years of professional experience
Work History
QC Specialist II
Cytek Biosciences
04.2024 - Current
- Performed quality control assays using flow cytometry to evaluate the performance of various antibodies.
- Developed greater familiarity with Cytek Aurora instruments and SpectroFlo software for flow cytometry applications.
- Received one-on-one mentorship in quality assurance, including incoming inspections, document control, and NCMR processing under the direct supervision of the site's Quality Engineer.
Scientist II - Antibody Conjugations
Thermo Fisher Scientific
04.2022 - 04.2024
- Conjugation of purified antibodies with different fluorescent dyes/formats
- Collaborated with crossfunctional teams to ensure successful transition of new Brilliant Ultra Violet format line into operations.
- Purification of conjugated antibodies via size-exclusion column chromatography
- Trackwise Process Owner (Quality Management System): problem notifications, investigation and root cause analysis and disposition of Problem Reports (PR)
- Participated in a Kaizen to address reoccurring quality issues in the AB Conjugation process
- Project Avant-Garde extended operations member (on-going) to validate and qualify an FPLC (automation instrument) for operations use
- Training Within the Industry (TWI) certified as of Q4 2022
Scientist I - Antibodies QC
Thermo Fisher Scientific
01.2021 - 04.2022
- Quality control testing of antibody lots via flow cytometry
- Worked with BD (Canto) and Cytek (Aurora) cytometers and analysis of flow cytometric data using FlowJo program
- Protein analysis via Western blots and SDS-PAGE
- BSL2 certified and experience in aseptic handling of select conjugated antibody formats
Sr. QC Technician - Gels
Thermo Fisher Scientific
09.2019 - 01.2021
- Quality control testing of polyacrylamide gels, protein standards, and buffers
- Real time analysis of data to ensure product meets company and department quality standards
- Dilutions, buffer and reagent preparation
- Participated in validations (component switch due to vendor discontinuation, equipment validation, new formulation validation).
- Agile (document management platform): Document updates of Work Instructions and Specification documents that pertained to the Gels Quality Control process
- "Project Rolex" extended operations member: performed tests and data collection for an ongoing "stickiness" issue in certain polyacrylamide gel types under R&D and Tech Ops supervision
Manufacturing Technician - Gels
Thermo Fisher Scientific
03.2019 - 09.2019
- Large scale formulation and in-process testing of solutions used in the manufacturing of different types of polyacrylamide gels
- GMP using aseptic techniques and proper handling/disposition of all chemicals used
- Monitored and maintained laboratory equipment and supplies necessary for production such as pH meters, refractometers, scales and balances
- Followed GMP protocols and Completed all batch records and manufacturing documents using GDP guidelines
Lab Assistant
BioLegend
12.2017 - 06.2018
- Assist in performing various bottle filling related tasks to include dispensing antibody solutions and capping and labeling of vials, tubes or bottles
- Verify batch records to ensure that information such as concentrations, lot numbers etc. are correct
- Set-up and prepare equipment for ongoing operations
- Clean and maintain work area and all lab equipment and supplies
Education
Bachelor of Science - Biological Sciences
California State University, San Marcos
01-2017
Skills
- Antibody manufacturing experience
- Document Review & Change Control
- Quality Management Systems and Tools (TrackWise, Propel)
- Deviation Investigation and CAPA Support
- Validation Support (Components & Equipment)
- Inventory Tracking & Material Management
Timeline
QC Specialist II
Cytek Biosciences
04.2024 - Current
Scientist II - Antibody Conjugations
Thermo Fisher Scientific
04.2022 - 04.2024
Scientist I - Antibodies QC
Thermo Fisher Scientific
01.2021 - 04.2022
Sr. QC Technician - Gels
Thermo Fisher Scientific
09.2019 - 01.2021
Manufacturing Technician - Gels
Thermo Fisher Scientific
03.2019 - 09.2019
Lab Assistant
BioLegend
12.2017 - 06.2018
Bachelor of Science - Biological Sciences
California State University, San Marcos
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