Longyu Zhang

Quality Control Department for first month:

• Learn methods and techniques for testing quality(enzyme activity); Work with colleague to test quality of products from other departments and sent data into record.

Genetic Engineering Department for rest of time:

• Learn mechanics and technique of molecular biology including: Preparation of E. coli competent cells; Transformation of plasmid DNA; Extraction of plasmid DNA; DNA detection by agarose gel electrophoresis; PCR gene amplification; DNA recombination; blue and white mottled screening test
  DNA digestion technology
• Learn to use Pichia multicopy expression vector kit for expression and isolation of recombinant proteins in Pichia pastoris containing multiple copies of GOI.
• Use genetic engineering techniques and work together with Lab group members, trying to increase enzyme activity of products.

The intern project introduce the detection technology of circulating tumor cells based on micro-nano multi-scale interface materials. The main contents include: (1) Design and preparation of multi-scale micro-nano interface materials; (2) Multi-scale interface materials for capturing circulating tumor cells in blood.

• Reading related articles, reviews, and books, understand principle of circulating tumor cell detection; understand preparation method of micro-nano materials.
• Make powerpoint presentation to tutors and other group members of project, including introduction multiple relevant reviews and articles (smart interface etc.), introduction of lab techniques.
• Learn how to prepare micro-nano interface materials.
• Independently prepare interface materials with different micro-nano structures.
• Learn how to culture circulating tumor cells.
• Capture and Characterize Circulating Tumor Cells.