Naomi Gonzales
Research Assistant II
Houston,TX
Summary
Seeking a challenging position as a Research Assistant II, leveraging my experience in conducting advanced research and data analysis. Committed to contributing to the advancement of knowledge and providing valuable insights. Skilled in experimental design, literature reviews, and report writing. Strong attention to detail, critical thinking, and problem-solving abilities.
Overview
8
8
years of professional experience
Work History
Research Assistant II
Baylor College of Medicine
01.2020 - Current
- Department of Molecular and Cellular Biology, Principal Investigator: Bert O’Malley, Supervisors: Meng Huan & David Lonard
- Aid in developing a mouse liver as a model to establish the 12h rhythmicity in relation to ER stress and metabolism and focus on the regulation of the 12h clock by SRC-3 and Xbp1s.
- Breeding and genotyping a doxycycline (Dox)-inducible, Kras G12D-driven PDAC mouse model
- Assist the co-investigator to perform metabolic analyses for Treg strategic plan in production of a cancer treatment
- Assist the co-investigator to perform single cell multiome RNA-Seq & ATAC-Seq and analyses
- Learn IHC protocols to make formalin fixed, paraffin embedded samples and stain slides with pathological antibody markers
- Maintain mouse colonies and perform routine genotyping and phenotyping following the IACUC guideline.
Research Assistant I
Baylor College of Medicine
01.2016 - 01.2020
- Department of Molecular and Cellular Biology, Principal Investigator: Brian York
- Assist co-investigator and graduate students in developing macrophage specific and liver specific knockout mouse models in determining the expression of CamKK2 in cancer development
- Assisted in designing and implementing experiments, demonstrating strong attention to detail and precision in data collection and analysis.
- Collaborated closely with a multidisciplinary team, effectively communicating research progress, results, and recommendations through regular meetings and written reports
- Managed and maintained research laboratory equipment and supplies, ensuring all materials were properly calibrated, organized, and available for use.
Education
B.A. in Biology -
University of St. Thomas
Houston, TX01.2004
Techniques And Skills
- Aseptic technique
- Mouse colony management and mouse genetics
- Primary hepatocyte isolation
- Tissue culture and manipulation
- Tissue collection and processing
- IHC/ICC/IF staining
- PCR, RT-PCR, qPCR
- Confocal and fluorescence microscopy
- Recombinant DNA cloning
- DNA and RNA isolation for downstream assays
- Western blotting
- DNA, RNA and protein isolation
- Protein expression and purification
- Flow cytometry analysis
- DNA pull down assay
- Nuclear extraction from tissues/cells
- Chromatin Immunoprecipitation
- ELISA
- Plasmid DNA transfection
- Luciferase reporter assay (plate reader & lumicycle)
- Genotyping troubleshooting and SOP development
- Advanced computing skills
- General laboratory management
- Development of SOPs
- Supply ordering
- Equipment maintenance
Recent Peer Reviewed Publications
- 12-h clock regulation of genetic information flow by XBP1s, Pan Y., Ballance H., Meng H., Gonzalez N., Kim Sam-Moon, Abdurehman L., York B., Chen X., Schnytzer Y., Levy O., Dacso C., McClung C., O’Malley B., Liu S., Zhu B., PLos Biology, 2020-01-14, 18, 1, e3000580
- Vitamin D Receptor Activation in Liver Macrophages Ameliorates Hepatic Inflammation, Steatosis, and Insulin Resistance in Mice, Dong B., Zhou Y., Wang W., Scott J., Kim KH., Sun Z., Guo Q., Lu Y., Gonzales N., Wu H., Hartig S., York R., Yang F., Moore D., Hepatology, 2020-05, 71, 5, 1559-1574
- Xbp1 links the 12-hour clock to NAFLD and regulation of membrane fluidity and lipid homeostasis, Huan M., Gonzales N., Lonard D., Putluri N., Zhu B., Dacso C., York B., O’Malley BW., Nature Communications, 2020-12, 11, 1
- Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism, Huan M., Gonzales N., Jung S. Y., Lu Y., Putluri N., Zhu B., Dacso C., Lonard D., O’Malley BW., Cell Reports, 2022-03, 38
Timeline
Research Assistant II
Baylor College of Medicine
01.2020 - Current
Research Assistant I
Baylor College of Medicine
01.2016 - 01.2020
B.A. in Biology -
University of St. Thomas
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