I am an ambitious, solution oriented and self motivated postdoctoral scholar with a strong background in Molecular Biology, Synthetic biology and microbiology with a deep understanding of DNA assemblies and genome editing tools, RNAi & CRISPR -Cas9 systems. I aspire to be a successful independent scientist focused working towards understanding and developing effective experimental designs, assays and user-friendly products/protocols to have a meaningful impact in real-world.
Molecular biology: DNA/RNA/plasmid extractions, Sanger sequencing, Cloning (Gibson assembly, golden gate), PCR, RT-PCR, qPCR, 5’ RACE PCR, RCA, ITS-PCR, Loop-mediated isothermal amplification for HLB diagnosis, designing duplex and multiplex PCR, In-vitro cleavage assay, PCR- restriction digestion based mutagenesis assay, northern and western blotting
Genome editing tools: Embryonic microinjections, CRISPR/Cas9 system, RNAi , sgRNA/dsRNA designing, synthesis and labeling, Piggyback vector design for multi DNA fragment assembly
Analytical Chemistry: Laser technology, Fluorometry, High performance liquid chromatography (HPLC), Liquid chromatography- mass spectrometry(LC-MS) Orbitrap
Cancer Biology: Ovarian cancer and primary cell culture, differential ultracentrifugation based Exosome isolation, exosomes mRNA and miRNA purification, PKH labeling of EVs, Time lapse Confocal microscopy, high-end fluorescence microscopy, Scratch assay
Microbiological Techniques: Bacteria, endophyte bacterial, Fungi (Phytohthora and Trichoderma) isolation, Identification, and maintenance, Antimicrobial profiling & Heavy metal sensitivity profiling of microbial strains, Basic Staining Techniques (Spores Staining, Negative Staining), Cell-viability assay, Sterilization Techniques, Centrifugation
Immunological Techniques: Immunological assay eg ELISA, RIA
Bioinformatics : BLAST, FASTA, ClustalW, Phylogenetic analysis & vector designing tools
Dr. Michael Riehle
(Post-doctoral Advisor)
Professor
Department of Entomology University of Arizona Forbes 410
1140 E. South Campus Drive Tucson,
Arizona 85721-0036
Phone: 520-626-8500
Fax: 520-621-1150
Email: mriehle@ag.arizona.edu
Dr. Judith Brown
(Post-doctoral Advisor)
Professor
School of Plant Sciences
1140 E. South Campus Dr.
Forbes Bld. #303
The University of Arizona Tucson,
AZ 85721, USA
Office Phone : 520-621-1402
Lab Phone : 520-621-1230
Cell Phone : 520-490-2109
Email: jbrown@ag.arizona.edu
Dr. Arti Shanware
(PhD Supervisor)
Assistant Professor,
Rajiv Gandhi Biotechnology Centre,
L.I.T Premises, R.T.M. Nagpur University
Nagpur - 440033 (M.S)
Ph No: +91-9823018963
E-mail: artishanware@gmail.com
Dr. Narendra Y. Kadoo
(Master’s advisor)
Scientist,
Plant Molecular Biology Group, Biochemistry Division,
National Chemical Laboratory,
Pune 411008, India
Ph: +91-20-25902247
Fax: +91-20-25902648
E-mail: ny.kadoo@ncl.res.in