Luigi Amoretti
Research Assistant
Scarsdale,NY
Summary
Detail-oriented research assistant with 6 years of laboratory bench experience at the Molecular Microbiome Core Facility responsible for the biobanking and sample processing of stool samples for NGS sequencing purposes. Highly motivated professional hoping to translate his laboratory skillset and project management skills within a more industrial setting to expand his impact on patients' lives. Strong background in nucleic acid extractions, PCR, qPCR, and library preparation for Illumina NGS platforms. As a part-time online graduate student, I demonstrate a level of time management, collaboration, and critical thinking that is shown in a professional setting as well.
Overview
7
7
years of professional experience
7
7
years of post-secondary education
Work History
Molecular Microbiome Facility Research Assistant
Memorial Sloan Kettering Cancer Center
New York, NY
09.2017 - Current
- Biobank inpatient and outpatient stool samples in compliance to IRB sample collection protocols, ongoing clinical trials, and multicenter consortium studies.
- Maintain sample and patient records within the Microsoft Access database system, as well as ensure electronic records accurately reflect the storage of samples within the biobank freezer system.
- Perform molecular biology protocols to isolate and sequence both metagenomic and 16S amplicon bacterial DNA from stool samples for Illumina NGS sequencing purposes: phenol-chloroform DNA extraction, RNA extraction, Qiagen QIAamp DNA purification, Qubit, PCR/qPCR, E-gel electrophoresis, Qiagen QIAquick PCR purification, Tapestation, Qiagen QIAseq 1-Step/KAPA Hyper library preparation.
- Coordinate with the Department of Lab Operations for the scheduling of preventative and corrective maintenance of equipment, such as qPCR/PCR thermocyclers, BSL-2 biosafety cabinets, and refrigerated centrifuges.
- Interpret laboratory QC results accurately to assess how to troubleshoot experiments and improve the quality/yield for future experiments.
Reproductive Biology Research Intern
Smithsonian's National Zoological Park
Washington, DC
01.2017 - 05.2017
- Experimented with a novel gamete preservation technique that involved the desiccation of ovarian tissue using a domestic cat model.
- Analyzed the ovarian cell morphology and viability using histological techniques such as tissue dissection, paraffin-embedding of tissue sections, microtome tissue sectioning, and H&E tissue staining.
- Engaged in seminars, discussion groups, and journal clubs about current experimental findings and issues in wildlife conservation reproductive research.
Immunology Research Intern
Albert Einstein College Of Medicine
Bronx, NY
06.2016 - 08.2016
- Assisted in experiments involving the construction and bacterial transformation of recombinant plasmids containing the HLA-B*3906 allele, which encodes for a class I Major Histocompatibility Complex protein associated with early onset of Type I Diabetes.
- Cooperated well with other post-docs and technicians to develop and test new experimental protocols in regard to the construction of recombinant plasmids.
- Prepared reagents and solutions following standard laboratory formulas and procedures.
Education
Master of Science - Biotechnology
Northeastern University
Boston, MA 01.2020 - Current
Bachelor of Science - Biology
University of Maryland - College Park
College Park, MD 09.2013 - 05.2017
Skills
Lab Equipment Management
Additional Information
Shouval, Roni et al. “Conditioning Regimens are Associated with Distinct Patterns of Microbiota Injury in Allogeneic Hematopoietic Cell Transplantation.” Clinical cancer research : an official journal of the American Association for Cancer Research vol. 29,1 (2023): 165-173. doi:10.1158/1078-0432.CCR-22-1254
Burgos da Silva, Marina et al. “Preservation of the fecal microbiome is associated with reduced severity of graft-versus-host disease.” Blood vol. 140,22 (2022): 2385-2397. doi:10.1182/blood.2021015352
Schluter, Jonas, et al. "The TaxUMAP atlas: efficient display of large clinical microbiome data reveals ecological competition involved in protection against bacteremia." bioRxiv (2022). doi: https://doi.org/10.1101/2022.09.27.509746
Rolling, Thierry et al. “Haematopoietic cell transplantation outcomes are linked to intestinal mycobiota dynamics and an expansion of Candida parapsilosis complex species.” Nature microbiology vol. 6,12 (2021): 1505-1515. doi:10.1038/s41564-021-00989-7
Yan, Jinyuan et al. “A compilation of fecal microbiome shotgun metagenomics from hematopoietic cell transplantation patients.” Scientific data vol. 9,1 219. 18 May. 2022, doi:10.1038/s41597-022-01302-9
Rolling, Thierry, et al. "Intestinal fungal dynamics and linkage to hematopoietic cell transplantation outcomes." medRxiv(2021). doi: https://doi.org/10.1101/2021.07.20.21260859
Liao, Chen et al. “Compilation of longitudinal microbiota data and hospitalome from hematopoietic cell transplantation patients.” Scientific data vol. 8,1 71. 2 Mar. 2021, doi:10.1038/s41597-021-00860-8
Becattini, Simone et al. “Rapid transcriptional and metabolic adaptation of intestinal microbes to host immune activation.” Cell host & microbe vol. 29,3 (2021): 378-393.e5. doi:10.1016/j.chom.2021.01.003
Schluter, Jonas, et al. "The gut microbiota is associated with immune cell dynamics in humans." Nature 588.7837 (2020): 303-307. doi:10.1038/s41586-020-2971-8
Becattini, Simone, et al. "Enhancing mucosal immunity by transient microbiota depletion." Nature communications 11.1 (2020): 1-13. doi:10.1038/s41467-020-18248-4
Peled, Jonathan U et al. “Microbiota as Predictor of Mortality in Allogeneic Hematopoietic-Cell Transplantation.” The New England journal of medicine vol. 382,9 (2020): 822-834. doi:10.1056/NEJMoa1900623
Zhai, Bing et al. “High-resolution mycobiota analysis reveals dynamic intestinal translocation preceding invasive candidiasis.” Nature medicine vol. 26,1 (2020): 59-64. doi:10.1038/s41591-019-0709-7
Lee, Pei-Chih, et al. "Influence of microwave-assisted dehydration on morphological integrity and viability of cat ovarian tissues: First steps toward long-term preservation of complex biomaterials at supra-zero temperatures." PLoS One14.12 (2019): e0225440. doi:10.1371/journal.pone.0225440
Stein-Thoeringer, C K et al. “Lactose drives Enterococcus expansion to promote graft-versus-host disease.” Science (New York, N.Y.) vol. 366,6469 (2019): 1143-1149. doi:10.1126/science.aax3760
Adhi, Fatima I., et al. "Pre-transplant fecal microbial diversity independently predicts critical illness after hematopoietic cell transplantation." Blood 134 (2019): 3264.
Kim, Sohn G et al. “Microbiota-derived lantibiotic restores resistance against vancomycin-resistant Enterococcus.” Naturevol. 572,7771 (2019): 665-669. doi:10.1038/s41586-019-1501-z
Peled, Jonathan U., et al. "Inferior survival after microbiota injury: A multicenter allo-HCT study." (2019): 7015-7015.
Peled, Jonathan U., et al. "Inferior survival after microbiota injury: A multicenter allo-HCT study." (2019): 7015-7015.
Timeline
Master of Science - Biotechnology
Northeastern University
01.2020 - Current
Molecular Microbiome Facility Research Assistant
Memorial Sloan Kettering Cancer Center
09.2017 - Current
Reproductive Biology Research Intern
Smithsonian's National Zoological Park
01.2017 - 05.2017
Immunology Research Intern
Albert Einstein College Of Medicine
06.2016 - 08.2016
Bachelor of Science - Biology
University of Maryland - College Park
09.2013 - 05.2017
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